Schetinger M R, Wyse A T, Da Silva L B, Barcellos C K, Dias R D, Sarkis J J
Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Brasil.
Biol Trace Elem Res. 1995 Dec;50(3):209-19. doi: 10.1007/BF02785411.
Aluminum chloride (AlCl3), a neurotoxic compound, inhibited ATP diphosphohydrolase activity of synaptosomes obtained from cerebral cortex of adult rats. The metal ion significantly inhibited ATPase and ADPase activities of the enzyme at all concentrations tested in vitro (0.01, 0.05, 0.5, 5, and 10 mM) in the presence of 1.5 mM calcium. When tested in the absence of Ca2+, and with increasing amounts of Al3+, enzyme activity remained below basal levels, suggesting that the trivalent cation Al3+ is not a substitute for the divalent cation Ca2+ in ATP-Ca2+ and ADP-Ca2+ complexes. The Al3+ inhibition was competitive with respect to Ca2+. The enzyme inhibition was reversed by the addition of deferoxamine (DFO). NaF significantly inhibited ATP diphosphohydrolase activity, and this inhibition was reversed by the addition of Ca2+ to the medium. Such inhibition was not potentiated by AlF4, which is an inhibitor of cation-transport ATPases.
氯化铝（AlCl3）是一种神经毒性化合物，它抑制了成年大鼠大脑皮层突触体的ATP二磷酸水解酶活性。在1.5 mM钙存在的情况下，该金属离子在体外测试的所有浓度（0.01、0.05、0.5、5和10 mM）下均显著抑制该酶的ATP酶和ADP酶活性。当在无Ca2+的情况下进行测试，且Al3+含量增加时，酶活性仍低于基础水平，这表明三价阳离子Al3+在ATP-Ca2+和ADP-Ca2+复合物中不能替代二价阳离子Ca2+。Al3+的抑制作用相对于Ca2+具有竞争性。通过添加去铁胺（DFO）可逆转酶的抑制作用。NaF显著抑制ATP二磷酸水解酶活性，并且通过向培养基中添加Ca2+可逆转这种抑制作用。AlF4（一种阳离子转运ATP酶的抑制剂）不会增强这种抑制作用。
