Sboĭchakov V B, Volkov I I, Badikov V D
Klin Lab Diagn. 1996 Jul-Aug(4):53-4.
A rapid method for measuring phosphatase activity of staphylococci has been developed. Russian 96-well polystyrene plates are used. 0.1 ml of 0.2% sodium phenolphthalein phosphate solution in 1% peptone water, pH 6.8 to 7.0, and 0.1 ml of the tested strain suspension in normal saline are put into wells and incubated for 5 h at 37 degrees C. The results are assessed after adding 0.05 ml of 2.5% ammonia spirit into each well. If the result is positive, the medium is colored raspberry-red, if negative-light-yellow. The method is simple, available, and cheap.
已开发出一种快速测定葡萄球菌磷酸酶活性的方法。使用俄罗斯的96孔聚苯乙烯板。将0.1毫升0.2%酚酞磷酸钠溶液加入1%蛋白胨水中（pH 6.8至7.0），再加入0.1毫升用生理盐水配制的受试菌株悬液，放入孔中，于37℃孵育5小时。向每个孔中加入0.05毫升2.5%氨溶液后评估结果。如果结果为阳性，培养基呈覆盆子红色；如果为阴性，则呈浅黄色。该方法简单、可行且成本低廉。
