Geary C, Stevens M
Med Lab Sci. 1989 Oct;46(4):291-4.
The ability of staphylococci to produce phosphatase is an important character in the identification of species in the genus. Current methods are slow and difficult to interpret. A test is described which uses p-nitrophenyl phosphate as the substrate. It was evaluated by testing 1035 reference and clinical strains, and also compared with an alternative method. All strains of Staphylococcus aureus and 83% of Staphylococcus epidermidis were phosphatase positive: all strains of Staphylococcus saprophyticus were negative. The method proved to be an accurate, reliable, economic and relatively rapid technique to detect phosphatase production.
葡萄球菌产生磷酸酶的能力是该属菌种鉴定中的一个重要特征。目前的方法耗时且难以解读。本文描述了一种以对硝基苯磷酸为底物的检测方法。通过对1035株参考菌株和临床菌株进行检测对其进行了评估，并与另一种方法进行了比较。所有金黄色葡萄球菌菌株和83%的表皮葡萄球菌菌株磷酸酶呈阳性：所有腐生葡萄球菌菌株均为阴性。该方法被证明是一种准确、可靠、经济且相对快速的检测磷酸酶产生的技术。
