Cloning and sequencing of the cDNA encoding beta-glucosidase 1 from Aspergillus aculeatus.
作者信息
Kawaguchi T, Enoki T, Tsurumaki S, Sumitani J, Ueda M, Ooi T, Arai M
机构信息
Department of Applied Biological Chemistry, University of Osaka Prefecture, Osaka, Japan.
出版信息
Gene. 1996 Sep 16;173(2):287-8. doi: 10.1016/0378-1119(96)00179-5.
PMID:8964516
Abstract
A cDNA was isolated from an Aspergillus aculeatus cDNA library using synthetic oligodeoxyribonucleotide mixtures that corresponded to the internal amino acid (aa) sequence of mature beta-glucosidase 1 (BGL1). Analysis of the nucleotide sequence of the cloned cDNA insert revealed a 2580-bp open reading frame (ORF) that encoded a 860-aa protein. The deduced aa sequence of the ORF shared sequence similarity with several BGL from other microorganisms.
摘要
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