来自黄孢原毛平革菌的一种新型纤维素结合β-葡萄糖苷酶的基因克隆与特性分析
Gene cloning and characterization of a novel cellulose-binding beta-glucosidase from Phanerochaete chrysosporium.
作者信息
Li B, Renganathan V
机构信息
Department of Biochemistry and Molecular Biology, Oregon Graduate Institute of Science and Technology, Portland 97291-1000, USA.
出版信息
Appl Environ Microbiol. 1998 Jul;64(7):2748-54. doi: 10.1128/AEM.64.7.2748-2754.1998.
Abstract
Analysis of a 2.4-kb cDNA of the cellulose-binding extracellular beta-glucosidase (CBGL) from Phanerochaete chrysosporium suggested that CBGL is organized into two domains, an N-terminal cellulose-binding domain and a C-terminal catalytic domain. Genomic sequence analysis suggested that cbgl is encoded by 30 exons. Southern analysis of DNA from homokaryotic cultures indicated that CBGL is encoded by two alleles, cbgl-1 and cbgl-2, of a single gene.
摘要
对来自黄孢原毛平革菌的纤维素结合细胞外β-葡萄糖苷酶（CBGL）的一个2.4kb cDNA进行分析表明，CBGL由两个结构域组成，一个N端纤维素结合结构域和一个C端催化结构域。基因组序列分析表明，cbgl由30个外显子编码。对同核体培养物的DNA进行Southern分析表明，CBGL由单个基因的两个等位基因cbgl-1和cbgl-2编码。
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