重缔合和杂交数据的最小二乘法分析程序。
A program for least squares analysis of reassociation and hybridization data.
作者信息
Pearson W R, Davidson E H, Britten R J
出版信息
Nucleic Acids Res. 1977 Jun;4(6):1727-37. doi: 10.1093/nar/4.6.1727.
Abstract
A computer program is described for the rapid calculation of least squares solutions for data fitted to different functions normally used in reassociation and hybridization kinetic measurements. The equations for the fraction not reacted as a function of Cot follow: First order, exp(-kCot); second order, (1+kCot)-1; variable order, (1+kCot)-n; approximate fraction of DNA sequence remaining single stranded, (1+kCot)-.44; and a function describing the pairing of tracer when the rate constant for the tracer (k) is distinct from the driver rate constant (kd): (formula: see text). Several components may be used for most of these functional forms. The standard deviations of the individual parameters at the solutions are calculated.
摘要
本文描述了一个计算机程序，用于快速计算与重缔合和杂交动力学测量中常用的不同函数拟合的数据的最小二乘解。未反应部分随Cot变化的方程如下：一级反应，exp(-kCot)；二级反应，(1 + kCot)-1；变级反应，(1 + kCot)-n；剩余单链DNA序列的近似分数，(1 + kCot)-0.44；以及当示踪剂的速率常数(k)与驱动剂速率常数(kd)不同时描述示踪剂配对的函数：(公式：见正文)。大多数这些函数形式可以使用几个组分。计算解处各个参数的标准差。
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