Ali B H, Bashir A K, Tanira M O
Desert and Marine Environment Research Centre, United Arab Emirates University, Al Ain.
Pharmacology. 1995 Dec;51(6):356-63. doi: 10.1159/000139347.
Crude ethanolic extract of Lawsonia inermis L. (0.25-2.0 g/kg) produced significant and dose-dependent anti-inflammatory, analgesic, and antipyretic effects in rats. The extract also produced significant increases in pentobarbitone-induced sleeping time. Using a liquid-liquid extraction procedure, the extract was fractionated into chloroform, butanol, and water fractions, and these were tested for the above activities. The butanol and chloroform fractions showed more potent anti-inflammatory, analgesic, and antipyretic effects than the crude extracts, while the aqueous extract showed significantly less effect. As compared with the other extracts, the butanolic extract (500 mg/kg) was the most effective in the analgesic test. From the chloroform extract, a pure compound was isolated and identified, using chromatographic and spectroscopic techniques, as 2-hydroxy-1,4-naphthaquinone (lawsone). The isolated compound was found to possess significant anti-inflammatory, analgesic, and antipyretic activity. It potentiated significantly the pentobarbitone-induced sleeping time. The anti-inflammatory effect of lawsone (500 mg/kg) was not significantly different from that of the reference drug phenylbutazone (100 mg/kg).
无患子叶乙醇粗提取物（0.25 - 2.0克/千克）对大鼠产生了显著且剂量依赖性的抗炎、镇痛和解热作用。该提取物还显著延长了戊巴比妥诱导的睡眠时间。采用液 - 液萃取法，将提取物分离为氯仿、丁醇和水相馏分，并对这些馏分进行上述活性测试。丁醇和氯仿馏分显示出比粗提取物更强的抗炎、镇痛和解热作用，而水提取物的作用则明显较弱。与其他提取物相比，丁醇提取物（500毫克/千克）在镇痛试验中效果最为显著。从氯仿提取物中，利用色谱和光谱技术分离鉴定出一种纯化合物，为2 - 羟基 - 1,4 - 萘醌（胡桃醌）。发现该分离出的化合物具有显著的抗炎、镇痛和解热活性。它显著延长了戊巴比妥诱导的睡眠时间。胡桃醌（500毫克/千克）的抗炎作用与参比药物保泰松（100毫克/千克）无显著差异。
