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本文引用的文献

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Comparison of Etest and National Committee for Clinical Laboratory Standards broth macrodilution method for antifungal susceptibility testing: enhanced ability to detect amphotericin B-resistant Candida isolates.用于抗真菌药敏试验的Etest法与美国国家临床实验室标准委员会肉汤稀释法的比较：增强检测两性霉素B耐药念珠菌分离株的能力。
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Correlation between in vitro and in vivo activity of antifungal agents against Candida species.抗真菌药物对念珠菌属的体外和体内活性之间的相关性。
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A randomized trial comparing fluconazole with amphotericin B for the treatment of candidemia in patients without neutropenia. Candidemia Study Group and the National Institute.一项比较氟康唑与两性霉素B治疗非中性粒细胞减少患者念珠菌血症的随机试验。念珠菌血症研究组与国立研究所。
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Selection of candidate quality control isolates and tentative quality control ranges for in vitro susceptibility testing of yeast isolates by National Committee for Clinical Laboratory Standards proposed standard methods.美国国家临床实验室标准委员会提出的标准方法用于酵母分离株体外药敏试验的候选质量控制菌株的选择及暂定质量控制范围。
J Clin Microbiol. 1994 Jul;32(7):1650-3. doi: 10.1128/jcm.32.7.1650-1653.1994.
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Detection of amphotericin B-resistant Candida isolates in a broth-based system.在基于肉汤的系统中检测对两性霉素B耐药的念珠菌分离株。
Antimicrob Agents Chemother. 1995 Apr;39(4):906-9. doi: 10.1128/AAC.39.4.906.
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Antifungal susceptibility testing of isolates from a randomized, multicenter trial of fluconazole versus amphotericin B as treatment of nonneutropenic patients with candidemia. NIAID Mycoses Study Group and the Candidemia Study Group.一项关于氟康唑与两性霉素B治疗非中性粒细胞减少念珠菌血症患者的随机多中心试验中分离菌株的抗真菌药敏试验。美国国立过敏和传染病研究所真菌病研究组及念珠菌血症研究组。
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7
Quality control guidelines for National Committee for Clinical Laboratory Standards recommended broth macrodilution testing of amphotericin B, fluconazole, and flucytosine.美国国家临床实验室标准委员会的质量控制指南推荐采用肉汤稀释法对两性霉素B、氟康唑和氟胞嘧啶进行检测。
J Clin Microbiol. 1995 May;33(5):1104-7. doi: 10.1128/jcm.33.5.1104-1107.1995.

用于测试念珠菌分离株对两性霉素B敏感性的抗生素培养基3的批次间变异性。

Lot-to-lot variability of antibiotic medium 3 used for testing susceptibility of Candida isolates to amphotericin B.

作者信息

Lozano-Chiu M, Nelson P W, Lancaster M, Pfaller M A, Rex J H

机构信息

Department of Internal Medicine, University of Texas Medical School, Houston 77030, USA.

出版信息

J Clin Microbiol. 1997 Jan;35(1):270-2. doi: 10.1128/jcm.35.1.270-272.1997.

DOI:10.1128/jcm.35.1.270-272.1997
PMID:8968923
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229554/
Abstract

We have previously shown that use of antibiotic medium 3 in the microdilution variant of the M27-T test method permits detection of amphotericin B-resistant Candida isolates. As this medium is not standardized and our initial work used only a single lot of antibiotic medium 3, we studied the lot-to-lot variability of three commercial lots of antibiotic medium 3 obtained from two sources. The MICs obtained with the new lots were lower than those of with the original lot, but the new lots still consistently separated putatively resistant and putatively susceptible isolates and this permits proposal of possible breakpoints for this assay system.

摘要

我们之前已经表明，在M27-T测试方法的微量稀释变体中使用抗生素培养基3可以检测出对两性霉素B耐药的念珠菌分离株。由于这种培养基未标准化，且我们最初的工作仅使用了一批抗生素培养基3，因此我们研究了从两个来源获得的三批商用抗生素培养基3的批次间变异性。新批次获得的最低抑菌浓度（MIC）低于原始批次，但新批次仍能始终如一地区分假定耐药和假定敏感的分离株，这使得能够为该检测系统提出可能的断点。