用于测试念珠菌分离株对两性霉素B敏感性的抗生素培养基3的批次间变异性。
Lot-to-lot variability of antibiotic medium 3 used for testing susceptibility of Candida isolates to amphotericin B.
作者信息
Lozano-Chiu M, Nelson P W, Lancaster M, Pfaller M A, Rex J H
机构信息
Department of Internal Medicine, University of Texas Medical School, Houston 77030, USA.
出版信息
J Clin Microbiol. 1997 Jan;35(1):270-2. doi: 10.1128/jcm.35.1.270-272.1997.
Abstract
We have previously shown that use of antibiotic medium 3 in the microdilution variant of the M27-T test method permits detection of amphotericin B-resistant Candida isolates. As this medium is not standardized and our initial work used only a single lot of antibiotic medium 3, we studied the lot-to-lot variability of three commercial lots of antibiotic medium 3 obtained from two sources. The MICs obtained with the new lots were lower than those of with the original lot, but the new lots still consistently separated putatively resistant and putatively susceptible isolates and this permits proposal of possible breakpoints for this assay system.
摘要
我们之前已经表明，在M27-T测试方法的微量稀释变体中使用抗生素培养基3可以检测出对两性霉素B耐药的念珠菌分离株。由于这种培养基未标准化，且我们最初的工作仅使用了一批抗生素培养基3，因此我们研究了从两个来源获得的三批商用抗生素培养基3的批次间变异性。新批次获得的最低抑菌浓度（MIC）低于原始批次，但新批次仍能始终如一地区分假定耐药和假定敏感的分离株，这使得能够为该检测系统提出可能的断点。
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