Lord V R, Cherwonogrodzky J W, Marcano M J, Melendez G
Instituto de Investigaciones Veterinarias, Laboratorio de Brucelosis, Maracay, Venezuela.
J Clin Microbiol. 1997 Jan;35(1):295-7. doi: 10.1128/jcm.35.1.295-297.1997.
A serological and bacteriological study was performed with sera taken from 2,228 swine from six states in Venezuela. None of the animals were vaccinated against brucellosis, and the prevalence of the disease varied from 5 to 89% on farms located in these states. Our studies indicated that the animals could be categorized into four groups depending on the degree of reactivity in serological tests. Brucella suis biovar 1 was isolated from the lymph nodes, spleens, and semen samples of seropositive animals and identified by oxidative metabolic techniques. B. suis could not be isolated from tissues of seronegative swine even from farms with cases of the disease (detected by serology). Results suggest that, although the immunodiffusion assay using Brucella melitensis B115 polysaccharide B or B. abortus 1119-3 O-polysaccharide could be useful in the detection of active infections, it is perhaps not as sensitive as some of the other standard serological tests used in this study for the detection of swine brucellosis.
对来自委内瑞拉六个州的2228头猪的血清进行了血清学和细菌学研究。这些动物均未接种布鲁氏菌病疫苗，在这些州的农场中，该病的患病率从5%到89%不等。我们的研究表明，根据血清学检测中的反应程度，这些动物可分为四组。从血清阳性动物的淋巴结、脾脏和精液样本中分离出猪布鲁氏菌生物变种1，并通过氧化代谢技术进行鉴定。即使在有病例的农场（通过血清学检测发现），也无法从血清阴性猪的组织中分离出猪布鲁氏菌。结果表明，尽管使用羊种布鲁氏菌B115多糖B或流产布鲁氏菌1119-3 O-多糖的免疫扩散试验可能有助于检测活动性感染，但它可能不如本研究中用于检测猪布鲁氏菌病的其他一些标准血清学检测敏感。
