采用Gen-Probe结核分枝杆菌直接检测法对非呼吸道标本中的结核分枝杆菌复合群进行直接检测。
Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test.
作者信息
Gamboa F, Manterola J M, Viñado B, Matas L, Giménez M, Lonca J, Manzano J R, Rodrigo C, Cardona P J, Padilla E, Domínguez J, Ausina V
机构信息
Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Barcelona, Spain.
出版信息
J Clin Microbiol. 1997 Jan;35(1):307-10. doi: 10.1128/jcm.35.1.307-310.1997.
Abstract
The Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (AMTDT) was adapted for the detection of Mycobacterium tuberculosis complex in 224 nonrespiratory specimens from 188 patients. The sensitivity and specificity of the AMTDT for such specimens, after resolution of discrepant results, were 85.7 and 100%, respectively. Pretreatment of nonrespiratory specimens with sodium dodecyl (lauryl) sulfate is mandatory to obtain consistent and reproducible AMTDT results. The use of 500 microliters of decontaminated specimen improves the sensitivity of the test. Because the AMTDT detects stable rRNA from noncultivable bacilli, it is not useful for monitoring patients receiving treatment.
摘要
Gen-Probe结核分枝杆菌直接扩增试验（AMTDT）用于检测188例患者的224份非呼吸道标本中的结核分枝杆菌复合群。在解决结果不一致的问题后，AMTDT对此类标本的敏感性和特异性分别为85.7%和100%。必须用十二烷基硫酸钠对非呼吸道标本进行预处理，以获得一致且可重复的AMTDT结果。使用500微升经去污处理的标本可提高检测的敏感性。由于AMTDT检测的是不可培养杆菌的稳定rRNA，因此它对监测正在接受治疗的患者没有用处。
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