Mizuno M, Masuda S, Takemaru K, Hosono S, Sato T, Takeuchi M, Kobayashi Y
Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Microbiology (Reading). 1996 Nov;142 ( Pt 11):3103-11. doi: 10.1099/13500872-142-11-3103.
As part of the Bacillus subtilis genome sequencing project, we have determined a 283 kb contiguous sequence from 210 degrees to 232 degrees of the B. subtilis genome. This region contains the 48 kb skin element which is excised during sporulation by a site-specific recombinase. In this region, 310 complete ORFs and one tRNA gene were identified: 66 ORFs have been sequenced and characterized previously by other workers, e.g. acc, ans, bfm, blt, bmr, comE, comG, dnaK, rpoD and sin operons; cwiA, gpr and lysA genes; many sporulation genes and operons, spo0A, spoIIA, spoIIM, spoiiP, spoIIIA, spoIIIC, spoIVB, spoIVCA, spoIVCB and spoVA, etc. The products of 84 ORFs were found to display significant similarity to proteins with known function in data banks, e.g., proteins involved in nucleotide metabolism, lipid biosynthesis, amino acid transport (ABC transporter), phosphate-specific transport, the glycine cleavage system, the two-component regulatory system, cell wall autolysis, ferric uptake and sporulation. However, the functions of more than half of the ORFs (52%, 160 ORFs) are still unknown. In the skin element containing 60 ORFs, 32 ORFs (53%) encode proteins which have significant homology to gene products of the B. subtilis temperate phage phi 105 and/or the defective phage PBSX.
作为枯草芽孢杆菌基因组测序项目的一部分，我们测定了枯草芽孢杆菌基因组从210°到232°的一段283 kb的连续序列。该区域包含48 kb的“皮肤”元件，在芽孢形成过程中由位点特异性重组酶切除。在这个区域，鉴定出了310个完整的开放阅读框（ORF）和一个tRNA基因：66个ORF先前已由其他研究人员测序并进行了表征，例如acc、ans、bfm、blt、bmr、comE、comG、dnaK、rpoD和sin操纵子；cwiA、gpr和lysA基因；许多芽孢形成基因和操纵子，如spo0A、spoIIA、spoIIM、spoiiP、spoIIIA、spoIIIC、spoIVB、spoIVCA、spoIVCB和spoVA等。发现84个ORF的产物与数据库中已知功能的蛋白质具有显著相似性，例如参与核苷酸代谢、脂质生物合成、氨基酸转运（ABC转运蛋白）、磷酸盐特异性转运、甘氨酸裂解系统、双组分调节系统、细胞壁自溶、铁摄取和芽孢形成的蛋白质。然而，超过一半的ORF（52%，即160个ORF）的功能仍然未知。在包含60个ORF的“皮肤”元件中，32个ORF（53%）编码的蛋白质与枯草芽孢杆菌温和噬菌体phi 105和/或缺陷噬菌体PBSX的基因产物具有显著同源性。
