Yamane K, Kumano M, Kurita K
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Microbiology (Reading). 1996 Nov;142 ( Pt 11):3047-56. doi: 10.1099/13500872-142-11-3047.
We determined a 146 kb contiguous sequence at the 25 degrees-36 degrees region of the Bacillus subtilis chromosome containing the amyE-srfA segment. Among the 113 ORFs identified, 33 are already known. functions were assigned to 38 ORFs by a search of non-redundant protein sequence data banks and those of 16 ORFs were suggested through significant similarity with reported sequences. The amino acid sequences of 13 of the ORfs were similar to proteins of unknown function of Escherichia coli, Haemophilus influenzae and other species. We did not find similarities for 29 ORFs to any known proteins. The 146 kb region is rich in enzymes (35 ORFs) related to the metabolism of low molecular mass compounds and five genes for surfactin production occupy about 26 kb of the region.
我们确定了枯草芽孢杆菌染色体25度-36度区域包含amyE-srfA片段的146 kb连续序列。在鉴定出的113个开放阅读框（ORF）中，33个已为人所知。通过搜索非冗余蛋白质序列数据库为38个ORF赋予了功能，另外16个ORF的功能则是通过与已报道序列的显著相似性推测出来的。其中13个ORF的氨基酸序列与大肠杆菌、流感嗜血杆菌及其他物种中功能未知的蛋白质相似。我们未发现29个ORF与任何已知蛋白质存在相似性。该146 kb区域富含与低分子量化合物代谢相关的酶（35个ORF），并且五个表面活性素产生基因占据了该区域约26 kb的长度。
