Ogasawara N, Nakai S, Yoshikawa H
Graduate School of Biological Sciences, Nara Advanced Institute of Science and Technology, Japan.
DNA Res. 1994;1(1):1-14. doi: 10.1093/dnares/1.1.1.
We have determined a 180 kb contiguous sequence in the replication origin region of the Bacillus subtilis chromosome. Open reading frames (ORF) in this region were unambiguously identified from the determined sequence, using criteria characteristic for the B. subtilis gene structure, i.e., starting with an ATG, GTG or TTG codon preceded by sequences complementary to the 3' end of the 16S rRNA. Four rRNA gene sets, 7 individual tRNA genes and 1 scRNA gene were identified, occupying 20 kb in total. In the remaining 160 kb region, 158 ORFs were identified, suggesting that 1 ORF is coded on average by 1 kb of DNA of the B. subtilis genome. Among the 158 ORFs, the functions of 48 ORFs were assigned and those of 11 ORFs are suggested through significant similarities to known proteins present in data banks. However, the functions of more than half of the ORFs (63%) remain to be determined.
我们已经确定了枯草芽孢杆菌染色体复制起始区域中一段180 kb的连续序列。利用枯草芽孢杆菌基因结构的特征标准，即从以16S rRNA 3'末端互补序列为前导的ATG、GTG或TTG密码子开始，从确定的序列中明确鉴定出该区域的开放阅读框（ORF）。鉴定出了4个rRNA基因集、7个单个tRNA基因和1个scRNA基因，总共占据20 kb。在其余160 kb区域中，鉴定出158个ORF，这表明枯草芽孢杆菌基因组平均每1 kb DNA编码1个ORF。在这158个ORF中，48个ORF的功能已被确定，11个ORF的功能通过与数据库中已知蛋白质的显著相似性得以推测。然而，超过一半（63%）的ORF功能仍有待确定。
