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枯草芽孢杆菌168染色体的ampS - nprE(124度 - 127度)区域:一段27 kb片段的测序及该区域几个基因的鉴定

The ampS-nprE (124 degrees-127 degrees) region of the Bacillus subtilis 168 chromosome: sequencing of a 27 kb segment and identification of several genes in the area.

作者信息

Winters P, Caldwell R, Enfield L, Ferrari E

机构信息

Genencor International, Inc., Department of Molecular Biology, Palo Alto, CA 94304, USA.

出版信息

Microbiology (Reading). 1996 Nov;142 ( Pt 11):3033-7. doi: 10.1099/13500872-142-11-3033.

Abstract

A stretch of DNA approximately 27 kb in length, adjacent to the nprE gene of Bacillus subtilis, has been sequenced. The sequenced fragment carries a total of 23 ORFs. Of these, 15 could be assigned probable functions based on homologies to characterized genes either in B. subtilis or in other organisms. The sequencing of this region has also allowed us to assign to this area adeC and strB, previously located on the other side of nprE, between nprE and the pyr operon.

摘要

对枯草芽孢杆菌nprE基因附近一段长度约为27 kb的DNA进行了测序。测序片段总共包含23个开放阅读框(ORF)。其中,15个可根据与枯草芽孢杆菌或其他生物体中已鉴定基因的同源性赋予可能的功能。该区域的测序还使我们能够将先前位于nprE另一侧、nprE与pyr操纵子之间的adeC和strB基因定位到该区域。

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