Barghouthi S, Guerdoud L M, Speert D P
Department of Paediatrics, University of British Columbia, Vancouver, Canada.
Am J Respir Crit Care Med. 1996 Dec;154(6 Pt 1):1788-93. doi: 10.1164/ajrccm.154.6.8970372.
Adherence of Pseudomonas aeruginosa to cells of the respiratory tract of patients with cystic fibrosis (CF) appears to be a necessary precondition for colonization and infection. To date no effective anti-adhesive strategy has been devised for preventing P. aeruginosa infection in these vulnerable hosts. The purpose of these studies was to evaluate the potential for preventing adhesion of P. aeruginosa to epithelial cells with dextran. Dextran (3,000-70,000 MW) inhibited adhesion of P. aeruginosa to buccal and A549 pulmonary epithelial cells; the 3,000 MW compound, at 10 mM was most inhibitory. Adhesion was inhibited optimally at pH 7.4 and was independent of charge; dextran and dextran sulfate were equally inhibitory. Dextran was most inhibitory if added to the epithelial cells before the P. aeruginosa; adhesion was reversed only minimally by adding dextran after the bacteria were bound. The inhibitory effect appeared to be nonspecific because other neutral polysaccharides (glycogen and mannan) were also inhibitory, dextran blocked attachment of other respiratory tract pathogens (Staphylococcus aureus, Group A streptococcus, and Haemophilus influenzae), and because dextran did not bind specifically to bacteria or to epithelial cells. Dextran is an inexpensive and nontoxic agent and may be useful in patients with CF to prevent colonization and infection with P. aeruginosa.
铜绿假单胞菌对囊性纤维化（CF）患者呼吸道细胞的黏附似乎是定植和感染的必要前提条件。迄今为止，尚未设计出有效的抗黏附策略来预防这些易感宿主中的铜绿假单胞菌感染。这些研究的目的是评估用葡聚糖预防铜绿假单胞菌黏附于上皮细胞的可能性。葡聚糖（分子量3000 - 70000）抑制了铜绿假单胞菌对颊黏膜和A549肺上皮细胞的黏附；10 mM的3000分子量化合物抑制作用最强。在pH 7.4时黏附抑制效果最佳，且与电荷无关；葡聚糖和硫酸葡聚糖的抑制作用相同。如果在铜绿假单胞菌之前添加到上皮细胞中，葡聚糖的抑制作用最强；在细菌黏附后添加葡聚糖，黏附逆转作用最小。这种抑制作用似乎是非特异性的，因为其他中性多糖（糖原和甘露聚糖）也有抑制作用，葡聚糖可阻止其他呼吸道病原体（金黄色葡萄球菌、A组链球菌和流感嗜血杆菌）的黏附，并且葡聚糖不会特异性结合细菌或上皮细胞。葡聚糖是一种廉价且无毒的药物，可能对CF患者预防铜绿假单胞菌的定植和感染有用。
