Department of Experimental and Clinical Pharmacology, College of Pharmacy, University of Minnesota, 717 Delaware St. SE, Minneapolis, MN 55414, USA.
Department of Microbiology & Immunology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt; Department of Pharmacy Practice, School of Pharmacy, Hampton University, Hampton, VA 23668, USA.
J Infect Public Health. 2014 Sep-Oct;7(5):436-44. doi: 10.1016/j.jiph.2014.01.009. Epub 2014 Jun 2.
Pseudomonas aeruginosa colonizes the lungs in cystic fibrosis (CF) and mechanically ventilated patients by binding to the cellular receptors on the surface of the lung epithelium. Studies have shown that blocking this interaction could be achieved with sub-minimum inhibitory concentrations of antibiotics such as ciprofloxacin. The development of bacterial resistance is a probable drawback of such an intervention. The use of natural extracts to interfere with bacterial adhesion and invasion has recently gained substantial attention and is hypothesized to inhibit bacterial binding and consequently prevent or reduce pathogenicity. This study used an A549 lung epithelial cell infection model, and the results revealed that a combination of aqueous cranberry extract with ciprofloxacin could completely prevent the adhesion and invasion of P. aeruginosa PAO1 compared to the untreated control. All of the natural extracts (cranberry, dextran, and soybean extracts) and ciprofloxacin showed a significant reduction (P<0.0001) in P. aeruginosa PAO1 adhesion to and invasion of lung epithelial cells relative to the control. The cranberry, dextran, and soybean extracts could substantially increase the anti-adhesion and anti-invasion effects of ciprofloxacin to the averages of 100% (P<0.0001), 80% (P<0.0001), and 60% (P<0.0001), respectively. Those extracts might result in a lower rate of the development of bacterial resistance; they are relatively safe and inexpensive agents, and utilizing such extracts, alone or in combination with ciprofloxacin, as potential anti-adhesion and anti-invasion remedies, could be valuable in preventing or reducing P. aeruginosa lung infections.
铜绿假单胞菌通过与肺上皮细胞表面的细胞受体结合，定植于囊性纤维化（CF）和机械通气患者的肺部。研究表明，用亚最小抑菌浓度的抗生素（如环丙沙星）阻断这种相互作用是可行的。这种干预措施的一个可能缺点是细菌耐药性的发展。最近，利用天然提取物干扰细菌黏附和侵袭的方法引起了广泛关注，并假设其可以抑制细菌结合，从而预防或减少致病性。本研究使用 A549 肺上皮细胞感染模型，结果表明，与未处理的对照组相比，含有水蔓越莓提取物的组合与环丙沙星一起可完全阻止铜绿假单胞菌 PAO1 的黏附和侵袭。与对照组相比，所有天然提取物（蔓越莓、葡聚糖和大豆提取物）和环丙沙星均显著降低了铜绿假单胞菌 PAO1 对肺上皮细胞的黏附和侵袭（P<0.0001）。蔓越莓、葡聚糖和大豆提取物可使环丙沙星的抗黏附和抗侵袭作用分别提高至平均 100%（P<0.0001）、80%（P<0.0001）和 60%（P<0.0001）。这些提取物可能会降低细菌耐药性的发展速度；它们是相对安全且廉价的药物，单独或与环丙沙星联合使用作为潜在的抗黏附和抗侵袭药物，可能有助于预防或减少铜绿假单胞菌肺部感染。
