Ayscough K R, Drubin D G
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3202, USA.
Annu Rev Cell Dev Biol. 1996;12:129-60. doi: 10.1146/annurev.cellbio.12.1.129.
Three of the most important questions concerning actin function are: (a) How does actin structure relate to actin function? (b) How does each of the numerous proteins that interact with actin contribute to actin cytoskeleton function in vivo? (c) How are the activities of these proteins regulated? Powerful molecular genetics combined with well-established biochemical techniques make the yeast Saccharomyces cerevisiae an ideal organism for studies aimed at answering these questions. The protein sequences and biochemical properties of actin and its interacting proteins and the pathways that regulate these interactions all appear to be conserved, indicating that principles elucidated from studies in yeast will apply to all eukaryotes. In this review, we highlight advances in our general understanding of actin properties, interactions with other proteins, and regulation of the actin cytoskeleton, derived from studies in the budding yeast S. cerevisiae.
(a) 肌动蛋白结构如何与肌动蛋白功能相关？(b) 众多与肌动蛋白相互作用的蛋白质中的每一种如何在体内对肌动蛋白细胞骨架功能做出贡献？(c) 这些蛋白质的活性如何被调节？强大的分子遗传学与成熟的生化技术相结合，使酿酒酵母成为旨在回答这些问题的研究的理想生物体。肌动蛋白及其相互作用蛋白质的蛋白质序列和生化特性以及调节这些相互作用的途径似乎都是保守的，这表明从酵母研究中阐明的原理将适用于所有真核生物。在这篇综述中，我们重点介绍了从对芽殖酵母酿酒酵母的研究中得出的，我们对肌动蛋白特性、与其他蛋白质的相互作用以及肌动蛋白细胞骨架调节的总体理解方面的进展。
