Azzi A, De Santis R, Ciappi S, Leoncini F, Sterrantino G, Marino N, Mazzotta F, Laszlo D, Fanci R, Bosi A
Institute of Microbiology, University of Florence, Italy.
J Neurovirol. 1996 Dec;2(6):411-6. doi: 10.3109/13550289609146907.
Peripheral blood leukocytes from immunocompetent and immunocompromised individuals were analyzed for human polyomarivus BK and JC DNA presence. A nested polymerase chain reaction which amplify the transcriptional control region of the genome of both viruses was employed. The immunocompromised patients included bone marrow transplantation recipients and AIDS patients. BKV sequences were detectable in 52.8-62.5% of the individuals included in this study, whereas the percentage of individuals with JCV sequences in peripheral blood lymphocytes varied from 38.8% to 50%. The frequency of reactivations of BKV and JCV were also determined by detection of shedding in urine of viral DNA. The highest frequency of reactivations of either BKV or JCV was demonstrable in the group of bone marrow transplantation recipients, but reactivations occurred also in immunocompetent individuals. JCV sequences amplified from urine samples showed a restriction pattern similar to the archetype one, whereas sequences obtained from lymphocytes showed rearranged pattern as well as archetype pattern. Finally all JCV sequences from cerebrospinal fluid seemed to be rearranged. These observations suggest that peripheral blood lymphocytes have a fundamental role in the persistence of polyomaviruses infection and in the dissemination at least of JCV within the organism allowing that rearranged variants, better adapted to grow in brain tissue, emerge.
对免疫功能正常和免疫功能低下个体的外周血白细胞进行分析，以检测人多瘤病毒BK和JC DNA的存在情况。采用巢式聚合酶链反应扩增这两种病毒基因组的转录控制区。免疫功能低下患者包括骨髓移植受者和艾滋病患者。在本研究纳入的个体中，52.8%至62.5%的个体可检测到BKV序列，而外周血淋巴细胞中带有JCV序列的个体百分比在38.8%至50%之间。还通过检测尿液中病毒DNA的排出情况来确定BKV和JCV的再激活频率。BKV或JCV再激活的最高频率在骨髓移植受者组中最为明显，但免疫功能正常的个体中也会发生再激活。从尿液样本中扩增出的JCV序列显示出与原型相似的限制性图谱，而从淋巴细胞中获得的序列则显示出重排图谱以及原型图谱。最后，来自脑脊液的所有JCV序列似乎都是重排的。这些观察结果表明，外周血淋巴细胞在多瘤病毒感染的持续存在以及至少在JCV在体内的传播中起着重要作用，使得更适应在脑组织中生长的重排变体得以出现。
