Wölfl S, Hanemann V, Saluz H P
Hans-Knöll-Institut für Naturstoff-Forschung, Department of Cell and Molecular Biology, Jena, Germany.
Yeast. 1996 Dec;12(15):1549-54. doi: 10.1002/(sici)1097-0061(199612)12:15<1549::aid-yea42>3.0.co;2-s.
The nucleotide sequence of a 26.7 kb DNA segment from the left arm of Saccharomyces cerevisiae chromosome IV is presented. An analysis of this segment revealed 11 open reading frames (ORFs) longer than 300 bp and one split gene. These ORFs include the genes encoding the large subunit of RNA polymerase II, the biotin apo-protein ligase, an ADP-ribosylation factor (ARF 2), the 'L35'-ribosomal protein, a rho GDP dissociation factor, and the sequence encoding the protein phosphatase 2A. Further sequence analysis revealed a short ORF encoding the ribosomal protein YL41B, an intron in a 5' untranslated region and an extended homology with another cosmid (X83276) located on the same chromosome. The potential biological relevance of these findings is discussed.
本文展示了来自酿酒酵母四号染色体左臂的一段26.7 kb DNA片段的核苷酸序列。对该片段的分析揭示了11个长度超过300 bp的开放阅读框（ORF）和一个断裂基因。这些ORF包括编码RNA聚合酶II大亚基、生物素脱辅基蛋白连接酶、ADP核糖基化因子（ARF 2）、“L35”核糖体蛋白、rho GDP解离因子的基因，以及编码蛋白磷酸酶2A的序列。进一步的序列分析揭示了一个编码核糖体蛋白YL41B的短ORF、一个位于5'非翻译区的内含子，以及与位于同一条染色体上的另一个黏粒（X83276）的延伸同源性。本文讨论了这些发现的潜在生物学意义。
