Putz U, Skehel P, Kuhl D
Center for Molecular Neurobiology (ZMNH), University of Hamburg, Germany.
Nucleic Acids Res. 1996 Dec 1;24(23):4838-40. doi: 10.1093/nar/24.23.4838.
A modification of the two-hybrid system is described for the in vivo reconstruction of specific RNA-protein interactions. In this tri-hybrid system, the DNA binding and transcription activation domains of the yeast transcriptional activator GAL4 are brought together via the interaction of recombinant fusion proteins with a recombinant RNA. The method provides a system for studying RNA-protein interactions with the genetic advantages of the two-hybrid system. It may be used to detect specific RNA-binding proteins or target RNAs from a library of cDNAs, or to analyse the structural specificity of identified RNA-protein interactions.
描述了一种用于体内重建特定RNA-蛋白质相互作用的双杂交系统的改进方法。在这个三杂交系统中，酵母转录激活因子GAL4的DNA结合域和转录激活域通过重组融合蛋白与重组RNA的相互作用而结合在一起。该方法提供了一个利用双杂交系统的遗传优势来研究RNA-蛋白质相互作用的系统。它可用于从cDNA文库中检测特定的RNA结合蛋白或靶RNA，或分析已鉴定的RNA-蛋白质相互作用的结构特异性。
