Kanoh N, Ogasawara H, Mohri D, Fukazawa K, Sakagami M
Department of Otolaryngology, Hyogo College of Medicine, Japan.
Acta Otolaryngol. 1996 Nov;116(6):824-7. doi: 10.3109/00016489609137933.
Dopamine is often used clinically for the treatment of patients with shock. In a previous study, the Na-KATPase (K-NPPase) activity of strial marginal cells was inhibited after the repeated in vivo administration of dopamine hydrochloride. In the present study, the K-NPPase activity of strial marginal cells was determined using a cerium-based cytochemistry after an in vitro incubation with dopamine. The enzyme reaction product was found in untreated normal strial marginal cells, but it was almost completely undetectable after an in vitro treatment with 10 mM dopamine. This finding suggested that dopamine directly inhibited the Na-KATPase activity of strial marginal cells, and that these cells might have a dopamine receptor.
多巴胺在临床上常被用于治疗休克患者。在先前的一项研究中，盐酸多巴胺反复经体内给药后，血管纹边缘细胞的钠钾ATP酶（K-NPP酶）活性受到抑制。在本研究中，血管纹边缘细胞经多巴胺体外孵育后，采用铈基细胞化学法测定其K-NPP酶活性。在未处理的正常血管纹边缘细胞中可发现酶反应产物，但用10 mM多巴胺进行体外处理后，几乎完全检测不到该产物。这一发现表明，多巴胺直接抑制血管纹边缘细胞的钠钾ATP酶活性，且这些细胞可能具有多巴胺受体。
