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嗜热甲基营养型甲醇芽孢杆菌限制修饰系统的特性分析

Characterization of a restriction-modification system of the thermotolerant methylotroph Bacillus methanolicus.

作者信息

Cue D, Lam H, Hanson R S, Flickinger M C

机构信息

Institute for Advanced Studies in Biological Process Technology, University of Minnesota, St. Paul 55108, USA.

出版信息

Appl Environ Microbiol. 1996 Mar;62(3):1107-11. doi: 10.1128/aem.62.3.1107-1111.1996.

DOI:10.1128/aem.62.3.1107-1111.1996
PMID:8975604
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC167875/
Abstract

We report the isolation of a restriction endonuclease, BmeTI, an isoschizomer of BclI, that recognizes the DNA sequence 5' TGATCA 3'. We also report that BmeTI sites are modified to TGm6ATCA. These findings provide the basis for devising strategies to prevent BmeTI restriction of any DNA introduced into Bacillus methanolicus.

摘要

我们报告了一种限制性内切酶BmeTI的分离情况，它是BclI的同裂酶，可识别DNA序列5'TGATCA 3'。我们还报告了BmeTI位点被修饰为TGm6ATCA。这些发现为设计策略以防止BmeTI对导入甲醇芽孢杆菌的任何DNA进行限制提供了基础。

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引用本文的文献

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Genetic manipulation of Bacillus methanolicus, a gram-positive, thermotolerant methylotroph.甲醇芽孢杆菌的基因操作，一种革兰氏阳性、耐热的甲基营养菌。
Appl Environ Microbiol. 1997 Apr;63(4):1406-20. doi: 10.1128/aem.63.4.1406-1420.1997.

本文引用的文献

1
Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.位点特异性甲基化对限制性内切核酸酶和DNA修饰甲基转移酶的影响。
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Assaying of organisms for the presence of restriction endonucleases.检测生物体中是否存在限制性内切酶。
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Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.改良的M13噬菌体克隆载体和宿主菌株：M13mp18和pUC19载体的核苷酸序列。
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A neomycin resistance gene cassette selectable in a single copy state in the Bacillus subtilis chromosome.一种在枯草芽孢杆菌染色体中以单拷贝状态可选择的新霉素抗性基因盒。
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L-lysine production at 50 degrees C by mutants of a newly isolated and characterized methylotrophic Bacillus sp.新分离鉴定的甲基营养型芽孢杆菌属菌株的突变体在50摄氏度下生产L-赖氨酸
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Cloning and nucleotide sequence of the gene coding for aspartokinase II from a thermophilic methylotrophic Bacillus sp.嗜热甲基营养芽孢杆菌中天冬氨酸激酶II编码基因的克隆与核苷酸序列分析
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Bacillus methanolicus sp. nov., a new species of thermotolerant, methanol-utilizing, endospore-forming bacteria.嗜甲醇芽孢杆菌新种，一种耐热、利用甲醇、形成芽孢的新型细菌。
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