嗜热甲基营养型甲醇芽孢杆菌限制修饰系统的特性分析
Characterization of a restriction-modification system of the thermotolerant methylotroph Bacillus methanolicus.
作者信息
Cue D, Lam H, Hanson R S, Flickinger M C
机构信息
Institute for Advanced Studies in Biological Process Technology, University of Minnesota, St. Paul 55108, USA.
出版信息
Appl Environ Microbiol. 1996 Mar;62(3):1107-11. doi: 10.1128/aem.62.3.1107-1111.1996.
Abstract
We report the isolation of a restriction endonuclease, BmeTI, an isoschizomer of BclI, that recognizes the DNA sequence 5' TGATCA 3'. We also report that BmeTI sites are modified to TGm6ATCA. These findings provide the basis for devising strategies to prevent BmeTI restriction of any DNA introduced into Bacillus methanolicus.
摘要
我们报告了一种限制性内切酶BmeTI的分离情况，它是BclI的同裂酶，可识别DNA序列5'TGATCA 3'。我们还报告了BmeTI位点被修饰为TGm6ATCA。这些发现为设计策略以防止BmeTI对导入甲醇芽孢杆菌的任何DNA进行限制提供了基础。
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