Ehret R, Baumann W, Brischwein M, Schwinde A, Stegbauer K, Wolf B
AG Medizinische Physik und Elektronenmikroskopie. Institut für Immunbiologie der Medizinischen Fakultät, Universität Freiburg, Germany.
Biosens Bioelectron. 1997;12(1):29-41. doi: 10.1016/0956-5663(96)89087-7.
A new method for on-line and real-time monitoring of concentration, growth and physiological state of cells in culture is described. This biosensor is based on impedance measurements of adherently growing cells on interdigitated electrode structures (IDES). The measurements can be performed for several days as there is no detectable electrical influence on the cells. The versatility of this new sensor is shown with some exemplary experiments. Cell density, growth and long-term behaviour of cells on the electrodes clearly change the impedance of the IDES. Both, the global influence of serum components (deprivation of foetal bovine serum) and the toxic effects of heavy metal ions (cadmium) result in changes of the sensor signal and can be visualized.
描述了一种用于在线实时监测培养细胞浓度、生长和生理状态的新方法。这种生物传感器基于对叉指电极结构（IDES）上贴壁生长细胞的阻抗测量。由于对细胞没有可检测到的电影响，测量可以进行数天。通过一些示例性实验展示了这种新型传感器的多功能性。电极上细胞的密度、生长和长期行为明显改变了IDES的阻抗。血清成分的整体影响（胎牛血清剥夺）和重金属离子（镉）的毒性作用都会导致传感器信号的变化，并且可以可视化。
