Ehret R, Baumann W, Brischwein M, Schwinde A, Wolf B
Institut für Immunbiologie, Universität Freiburg, Germany.
Med Biol Eng Comput. 1998 May;36(3):365-70. doi: 10.1007/BF02522485.
Critical parameters to be assessed in cell culture are the number of viable cells and cell viability. Growth, product formation, toxicity effects and the overall success of cell culture can depend largely on these. With interdigitated electrode structures (IDES) adherent cells are cultured directly on a pair of interdigitated electrodes, and the impedance of the system gives insight into the adhesive behaviour of the cells. The signal is influenced by the changes in number, growth and morphological behaviour of adherently growing cells, mainly owing to the insulating effects of the cell membranes. Five different cell lines are used, and their divergent behaviour is monitored over a period of four days, from inoculation of the cells to killing of the cells at the end of the experiments. Even when the cells from close monolayers, great fluctuations in the impedance signal can be observed. Nevertheless, for a more complete description of cellular systems, other parameters, such as acidification and respiration, have to be included in the measuring system.
细胞培养中需要评估的关键参数是活细胞数量和细胞活力。细胞生长、产物形成、毒性作用以及细胞培养的总体成功在很大程度上可能取决于这些参数。利用叉指电极结构（IDES），贴壁细胞直接在一对叉指电极上进行培养，系统的阻抗可深入了解细胞的粘附行为。该信号受贴壁生长细胞数量、生长和形态行为变化的影响，主要是由于细胞膜的绝缘作用。使用了五种不同的细胞系，并在四天时间内监测它们的不同行为，从细胞接种到实验结束时细胞死亡。即使细胞形成紧密单层，也能观察到阻抗信号的巨大波动。然而，为了更全面地描述细胞系统，测量系统中还必须纳入其他参数，如酸化和呼吸作用。
