Chen K S, Paladugu A, Aldaz C M, Gould M N
Department of Human Oncology, University of Wisconsin, Madison 53792, USA.
Cytogenet Cell Genet. 1996;74(4):277-80. doi: 10.1159/000134434.
Rat beta-casein gene expression is controlled in part by the positively acting factor Stat5 and the negatively acting factor Yy1. Partial cDNAs encoding the SH2 domain of rat Stat5a and Stat5b and the zinc finger domain of rat Yy1 have been cloned by an RT-PCR based approach. The nucleotide sequences encoding the SH2 domain of rat Stat5a and Stat5b are 75% homologous. The rat Yy1 cDNA is 95% homologous to its human and mouse counterparts in the zinc finger domain-encoding region. Using fluorescence in situ hybridization, we have localized Stat5a to rat chromosome band 10q32.1 and Yy1 to rat chromosome band 6q32.
大鼠β-酪蛋白基因的表达部分受正向作用因子Stat5和负向作用因子Yy1的调控。通过基于逆转录聚合酶链反应（RT-PCR）的方法，已克隆出编码大鼠Stat5a和Stat5b的SH2结构域以及大鼠Yy1的锌指结构域的部分互补DNA（cDNA）。编码大鼠Stat5a和Stat5b的SH2结构域的核苷酸序列具有75%的同源性。大鼠Yy1 cDNA在锌指结构域编码区与人类和小鼠的对应序列具有95%的同源性。利用荧光原位杂交技术，我们已将Stat5a定位到大鼠染色体带10q32.1，将Yy1定位到大鼠染色体带6q32。
