一种与非洲爪蟾转录因子IIIA高度同源的新型人类基因（GTF3A）的分子克隆、特性分析及染色体定位

Molecular cloning, characterization, and chromosomal mapping of a novel human gene (GTF3A) that is highly homologous to Xenopus transcription factor IIIA.

作者信息

Arakawa H, Nagase H, Hayashi N, Ogawa M, Nagata M, Fujiwara T, Takahashi E, Shin S, Nakamura Y

机构信息

Department of Biochemistry, Cancer Institute, Tokyo, Japan.

出版信息

Cytogenet Cell Genet. 1995;70(3-4):235-8. doi: 10.1159/000134041.

DOI:10.1159/000134041

PMID:7789179

Abstract

We have isolated a novel human cDNA that is highly related to Xenopus transcription factor IIIA (TFIIIA). This clone contains an open reading frame of 1,269 nucleotides encoding 423 amino acids, including nine repeats of the Cys2His2-type of zinc-finger domain. A comparison of its sequence with Xenopus TFIIIA revealed 63% identity in nucleic acids and 58% identity in amino acids over a large portion of the gene and predicted peptide, indicating that the human homologue is likely to function as a transcription factor. The zinc-finger domains of the predicted protein also showed homology with those of human genes such as WT1, transcriptional repressor YY1, and MYC-associated zinc-finger protein (MAZ). Northern analysis showed expression in various tissues examined. The human TFIIIA gene (GTF3A) was localized to chromosome band 13q12.3-->q13.1 by fluorescent in situ hybridization (FISH).

摘要

我们分离出了一种与非洲爪蟾转录因子IIIA（TFIIIA）高度相关的新型人类cDNA。该克隆包含一个1269个核苷酸的开放阅读框，编码423个氨基酸，其中包括九个Cys2His2型锌指结构域重复序列。将其序列与非洲爪蟾TFIIIA进行比较，发现在该基因和预测肽的大部分区域，核酸一致性为63%，氨基酸一致性为58%，这表明人类同源物可能作为转录因子发挥作用。预测蛋白的锌指结构域也与WT1、转录抑制因子YY1和MYC相关锌指蛋白（MAZ）等人类基因的锌指结构域具有同源性。Northern分析显示在所检测的各种组织中均有表达。通过荧光原位杂交（FISH）将人类TFIIIA基因（GTF3A）定位到染色体带13q12.3→q13.1。