Yao Y L, Dupont B R, Ghosh S, Fang Y, Leach R J, Seto E
H. Lee Moffitt Cancer Center and Research Institute, Department of Microbiology and Immunology, College of Medicine, University of South Florida, 12902 Magnolia Drive, Tampa, FL 33612, USA.
Nucleic Acids Res. 1998 Aug 15;26(16):3776-83. doi: 10.1093/nar/26.16.3776.
Yin Yang 1 (YY1) is a protein that activates and represses transcription of a large number of cellular and viral genes. In addition, studies suggest that YY1 may play an important role in development and differentiation. Here, we report the isolation and analysis of a YY1 genomic clone from a lambda human liver library. Fluorescence in situ hybridization with the YY1 clone has localized the YY1 gene to chromosome 14 band q32. A major YY1 gene transcription initiation site has been mapped to 478 bp upstream of the ATG translation start site. The proximal promoter contains multiple Sp1 transcription factor binding sites but lacks a consensus TATA or CCAAT box. Transient transfections and detailed deletion analyses localized the promoter to no more than 277 bp upstream from the major transcription start site. Finally, we have found that overexpression of the adenovirus E1A protein represses expression of a reporter gene directed by the YY1 promoter.
阴阳1（YY1）是一种可激活和抑制大量细胞及病毒基因转录的蛋白质。此外，研究表明YY1可能在发育和分化过程中发挥重要作用。在此，我们报告了从λ噬菌体人肝脏文库中分离和分析YY1基因组克隆的过程。用YY1克隆进行荧光原位杂交已将YY1基因定位到14号染色体q32带。一个主要的YY1基因转录起始位点已被定位到ATG翻译起始位点上游478 bp处。近端启动子包含多个Sp1转录因子结合位点，但缺乏共有TATA或CCAAT框。瞬时转染和详细的缺失分析将启动子定位到主要转录起始位点上游不超过277 bp处。最后，我们发现腺病毒E1A蛋白的过表达会抑制由YY1启动子驱动的报告基因的表达。
