Shahmanesh M, Pandit P G, Round R
Department of Genitourinary Medicine, Whittall Street Clinic, Birmingham, UK.
Genitourin Med. 1996 Oct;72(5):362-4. doi: 10.1136/sti.72.5.362.
A pilot feasibility study to investigate the local immune response in patients with non-gonococcal urethritis (NGU).
Urethral lymphocytes were extracted from first void urine (FVU) of patients with non-gonococcal urethritis and gonorrhoea using magnetic beads coated with antibody against either the pan-T cell marker CD2 or to CD4. The CD2+ and CD4+ lymphocyte content of FVU were compared between chlamydia-positive and chlamydia-negative and between first and subsequent attacks of NGU.
Median CD2+ lymphocyte content of FVU was significantly higher in cases of gonorrhoea and chlamydia-positive urethritis than in chlamydia-negative NGU. Median CD2+ lymphocyte content of FVU in chlamydia-negative NGU was 15.6 x 10(4), (n = 14 range 3.2-111) which was significantly lower than in chlamydia positive NGU (35.2 x 10(4), n = 18, range 7.4-390 p = 0.037) and gonorrhoea (67.7 x 10(4), n = 8 range 13-501, p = 0.019). Comparing the first with subsequent attacks of NGU, CD2+ cell numbers were greater in those presenting with their first episode of chlamydia-negative NGU (21.8 x 10(4), range 5.6-111) compared with those who had experienced NGU before (12.3 x 10(4), range 3.2-20.4, p = 0.033), but not in those presenting with chlamydia-positive NGU. There was no difference in CD4+ cells between any of the groups.
The lower total lymphocyte content of subsequent attacks of chlamydia-negative NGU compared with the first attack chlamydia-positive and negative NGU suggests that a different aetiology may be operating in some of these patients. Sufficient lymphocytes can be isolated from the urethra in patients with NGU for studies of lymphocyte subsets to be carried out.
一项初步可行性研究，旨在调查非淋菌性尿道炎（NGU）患者的局部免疫反应。
使用包被有抗泛T细胞标志物CD2或CD4抗体的磁珠，从非淋菌性尿道炎和淋病患者的首次晨尿（FVU）中提取尿道淋巴细胞。比较衣原体阳性和衣原体阴性患者以及非淋菌性尿道炎首次发作和后续发作时FVU中CD2+和CD4+淋巴细胞含量。
淋病和衣原体阳性尿道炎患者FVU中CD2+淋巴细胞含量中位数显著高于衣原体阴性非淋菌性尿道炎患者。衣原体阴性非淋菌性尿道炎患者FVU中CD2+淋巴细胞含量中位数为15.6×10⁴（n = 14，范围3.2 - 111），显著低于衣原体阳性非淋菌性尿道炎患者（35.2×10⁴，n = 18，范围7.4 - 390，p = 0.037）和淋病患者（67.7×10⁴，n = 8，范围13 - 501，p = 0.019）。比较非淋菌性尿道炎首次发作和后续发作情况，衣原体阴性非淋菌性尿道炎首次发作患者的CD2+细胞数（21.8×10⁴，范围5.6 - 111）高于既往有非淋菌性尿道炎病史的患者（12.3×10⁴，范围3.2 - 20.4，p = 0.033），而衣原体阳性非淋菌性尿道炎患者则无此差异。各组之间CD4+细胞无差异。
衣原体阴性非淋菌性尿道炎后续发作时的总淋巴细胞含量低于首次发作时的衣原体阳性和阴性非淋菌性尿道炎，这表明部分此类患者可能存在不同的病因。非淋菌性尿道炎患者尿道中可分离出足够的淋巴细胞用于淋巴细胞亚群研究。
