Gao A C, Isaacs J T
Johns Hopkins Oncology Center, James Buchanan Brady Urological Institute, Department of Urology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.
Prostate. 1996 Dec;29(6):395-8. doi: 10.1002/(SICI)1097-0045(199612)29:6<395::AID-PROS8>3.0.CO;2-7.
Homeobox genes encode transcription factors involved in the genetic control of normal development and differentiation, as well as in malignant transformation. To begin to assess the possible role of homeobox genes in prostatic cell carcinogenesis, we surveyed initially for expression of homeobox-containing genes in the TSU-PR1 cell line.
This was performed by RT-PCR using degenerate oligodeoxyribonucleotide primers to the homeobox-binding sequence to generate partial cDNAs which were cloned and sequenced.
Using this method, expression of 14 members of homeobox-containing genes were detected in TSU-PR1 cells. All of these expressed genes correspond to previously identified homeobox genes located within the HOXA, B, C, and D clusters. We further examined the expression of these homeobox genes in different human prostatic cell lines by using whole cDNA slot blot and Northern blot analysis. One of the sequences corresponding to the human GBX2 homeobox gene is overexpressed in TSU-PR1, LNCaP, PC-3, and DU145 metastatic prostate cell lines relative to the normal prostate.
Our results suggest that the homeobox gene GBX2 may participate in metastatic progression in prostatic cancer.
同源框基因编码参与正常发育、分化以及恶性转化遗传控制的转录因子。为了初步评估同源框基因在前列腺细胞癌变中的可能作用，我们首先对TSU-PR1细胞系中含同源框基因的表达情况进行了调查。
采用逆转录聚合酶链反应（RT-PCR），使用与同源框结合序列对应的简并寡脱氧核糖核苷酸引物来生成部分互补DNA（cDNA），然后进行克隆和测序。
使用该方法，在TSU-PR1细胞中检测到14个含同源框基因成员的表达。所有这些表达的基因都对应于先前在HOXA、B、C和D簇中鉴定出的同源框基因。我们通过全cDNA斑点印迹和Northern印迹分析进一步检测了这些同源框基因在不同人前列腺细胞系中的表达。相对于正常前列腺，与人类GBX2同源框基因对应的一个序列在TSU-PR1、LNCaP、PC-3和DU145转移性前列腺癌细胞系中过表达。
我们的结果表明，同源框基因GBX2可能参与前列腺癌的转移进展。
