Katagiri T, Ozaki K, Fujiwara T, Shimizu F, Kawai A, Okuno S, Suzuki M, Nakamura Y, Takahashi E, Hirai Y
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan.
Cytogenet Cell Genet. 1996;74(1-2):90-5. doi: 10.1159/000134389.
From a human fetal-brain cDNA library we isolated a novel human cDNA, termed human adducin-like 70 (gene symbol ADDL), whose predicted amino acid sequence showed a high degree of homology to adducins. This cDNA clone (ADDL), which contained an open reading frame of 2,022 nucleotides encoding 674 amino acids, revealed 54%, 53%, and 59% identity in predicted amino acid sequence with alpha and beta components of human adducin and rat adducin 63, respectively. Human adducin-like 70 is likely to play an important role in the skeletal organization of the cell membrane. Northern blot analysis indicated ubiquitous expression of this gene in adult human tissues. We localized the gene to chromosome bands 10q24.2-->q24.3 by fluorescence in situ hybridization (FISH).
我们从一个人类胎儿脑cDNA文库中分离出一个新的人类cDNA，命名为人类类内收蛋白70（基因符号ADDL），其预测的氨基酸序列与内收蛋白具有高度同源性。这个cDNA克隆（ADDL）包含一个2022个核苷酸的开放阅读框，编码674个氨基酸，其预测的氨基酸序列与人类内收蛋白的α和β亚基以及大鼠内收蛋白63分别有54%、53%和59%的同一性。人类类内收蛋白70可能在细胞膜的骨架组织中发挥重要作用。Northern印迹分析表明该基因在成人组织中普遍表达。我们通过荧光原位杂交（FISH）将该基因定位到染色体10q24.2→q
