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鹰嘴豆(Cicer arietinum L.)离体生长茎尖外植体的生根与植株建立

Rooting and establishment of in vitro grown shoot tip explants of chickpea (Cicer arietinum L.).

作者信息

Polisetty R, Patil P, Deveshwar J J, Khetarpal S, Chandra R

机构信息

Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, India.

出版信息

Indian J Exp Biol. 1996 Aug;34(8):806-9.

PMID:8979490
Abstract

Shoot tip explants of 5.chickpea varieties were evaluated for efficiency of rooting using MS basal medium (1/4, 1/2 and full strength). Maximum rooting was observed in 3 varieties when cultured in MS medium with 1/4 strength. Plantlets thus developed, were further cultured in Hoagland solution (1/4 strength) for 15-20 days which enhanced root number and root length. Such plantlets established well in vermiculite/soil.

摘要

使用MS基本培养基(1/4强度、1/2强度和全强度)对5个鹰嘴豆品种的茎尖外植体进行生根效率评估。当在1/4强度的MS培养基中培养时,在3个品种中观察到最大生根量。如此培育出的幼苗在霍格兰溶液(1/4强度)中进一步培养15 - 20天,这增加了根的数量和长度。这些幼苗在蛭石/土壤中生长良好。

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