Davis Samuel, Aldrich Thomas H, Jones Pamela F, Acheson Ann, Compton Debra L, Jain Vivek, Ryan Terence E, Bruno Joanne, Radziejewski Czeslaw, Maisonpierre Peter C, Yancopoulos George D
Regeneron Pharmaceuticals, Inc., Tarrytown, New York 10591, USA.
Cell. 1996 Dec 27;87(7):1161-9. doi: 10.1016/s0092-8674(00)81812-7.
TIE2 is a receptor-like tyrosine kinase expressed almost exclusively in endothelial cells and early hemopoietic cells and required for the normal development of vascular structures during embryogenesis. We report the identification of a secreted ligand for TIE2, termed Angiopoietin-1, using a novel expression cloning technique that involves intracellular trapping and detection of the ligand in COS cells. The structure of Angiopoietin-1 differs from that of known angiogenic factors or other ligands for receptor tyrosine kinases. Although Angiopoietin-1 binds and induces the tyrosine phosphorylation of TIE2, it does not directly promote the growth of cultured endothelial cells. However, its expression in close proximity with developing blood vessels implicates Angiopoietin-1 in endothelial developmental processes.
TIE2是一种类受体酪氨酸激酶，几乎只在内皮细胞和早期造血细胞中表达，是胚胎发育过程中血管结构正常发育所必需的。我们报告了使用一种新的表达克隆技术鉴定出一种TIE2的分泌配体，称为血管生成素-1，该技术涉及在COS细胞中进行细胞内捕获和配体检测。血管生成素-1的结构不同于已知的血管生成因子或受体酪氨酸激酶的其他配体。尽管血管生成素-1能结合并诱导TIE2的酪氨酸磷酸化，但它并不直接促进培养的内皮细胞生长。然而，它在发育中的血管附近的表达表明血管生成素-1参与了内皮发育过程。
