Greenwood J, Pryce G, Devine L, Male D K, dos Santos W L, Calder V L, Adamson P
Department of Clinical Ophthalmology, University College London, UK.
J Neuroimmunol. 1996 Dec;71(1-2):51-63. doi: 10.1016/s0165-5728(96)00130-0.
In the central nervous system the blood-brain and blood-retinal barriers (BBB and BRB respectively) are instrumental in maintaining homeostasis of the neural parenchyma and controlling leucocyte traffic. These cellular barriers are formed primarily by the vascular endothelium of the brain and retina although in the latter the pigmented epithelial cells also form part of the barrier. From primary cultures of rat brain endothelium, retinal endothelium and retinal pigment epithelium (RPE) we have generated temperature sensitive SV40 large T immortalised cell lines. Clones of brain (GP8.3) and retinal (JG2.1) endothelia and RPE (LD7.4) have been derived from parent lines that express the large T antigen at the permissive temperature. The endothelial cell (EC) lines expressed P-glycoprotein, GLUT-1, the transferrin receptor, von Willebrand factor and the RECA-1 antigen and exhibited high affinity uptake of acetylated LDL and stained positive with the lectin Griffonia simplicifolia. The RPE cell line was positive for cytokeratins and for the rat RPE antigen RET-PE2. All the cell lines expressed major histocompatibility complex (MHC) class 1 and intercellular adhesion molecule (ICAM)-1 constitutively and could be induced to express MHC class II and vascular cell adhesion molecule (VCAM)-1 following cytokine activation. The EC also expressed platelet endothelial cell adhesion molecule (PECAM)-1. Monolayers of these cells could support the migration of antigen-specific T cell lines. The generation of immortalised cell lines derived from the rat BBB and BRB should prove to be useful tools for the study of these specialised cellular barriers.
在中枢神经系统中，血脑屏障和血视网膜屏障（分别为BBB和BRB）有助于维持神经实质的内环境稳定并控制白细胞运输。这些细胞屏障主要由脑和视网膜的血管内皮形成，不过在视网膜中，色素上皮细胞也构成屏障的一部分。我们从大鼠脑内皮、视网膜内皮和视网膜色素上皮（RPE）的原代培养物中生成了温度敏感的SV40大T抗原永生化细胞系。脑内皮（GP8.3）、视网膜内皮（JG2.1）和RPE（LD7.4）的克隆源自于在允许温度下表达大T抗原的亲本细胞系。内皮细胞（EC）系表达P-糖蛋白、葡萄糖转运蛋白1、转铁蛋白受体、血管性血友病因子和RECA-1抗原，对乙酰化低密度脂蛋白表现出高亲和力摄取，并被凝集素单叶豆凝集素染色呈阳性。RPE细胞系细胞角蛋白和大鼠RPE抗原RET-PE2呈阳性。所有细胞系均组成性表达主要组织相容性复合体（MHC）I类和细胞间黏附分子（ICAM）-1，细胞因子激活后可被诱导表达MHC II类和血管细胞黏附分子（VCAM）-1。EC还表达血小板内皮细胞黏附分子（PECAM）-1。这些细胞的单层可支持抗原特异性T细胞系的迁移。源自大鼠BBB和BRB的永生化细胞系的产生应被证明是研究这些特殊细胞屏障的有用工具。
