Labow R S, Erfle D J, Santerre J P
Cardiovascular Devices Division, University of Ottawa Heart Institute, ON, Canada.
Biomaterials. 1996 Dec;17(24):2381-8. doi: 10.1016/s0142-9612(96)00088-9.
Human neutrophil elastase (HNE) and porcine pancreatic elastase (PPE) were incubated with two radiolabelled model poly(urethane), a poly(ester-urea-urethane) containing [14C]toluene diisocyanate ([14C]TDI), poly(caprolactone)(PCL) and ethylenediamine (ED), and a poly(ether-urea-urethane) containing [14C]TDI, poly(tetramethylene oxide) (PTMO) and ED. Ten-fold more radioactive carbon was released when PPE was incubated with [14C]TDI/PCL/ED than when HNE was used. The PPE-induced radioactive carbon release was significantly reduced by a specific elastase inhibitor. Ten-fold less radioactive carbon was released when [14C]TDI/PTMO/ED was incubated with PPE as compared to [14C]TDI/PCL/ED. Since neutrophils, which contain elastolytic activity, are present during the inflammatory response, the stability of biomaterials used in implanted devices may be affected.
将人中性粒细胞弹性蛋白酶（HNE）和猪胰弹性蛋白酶（PPE）与两种放射性标记的模型聚氨酯一起孵育，一种是含有[14C]甲苯二异氰酸酯（[14C]TDI）、聚己内酯（PCL）和乙二胺（ED）的聚（酯 - 脲 - 聚氨酯），另一种是含有[14C]TDI、聚四氢呋喃（PTMO）和ED的聚（醚 - 脲 - 聚氨酯）。当PPE与[14C]TDI/PCL/ED孵育时，释放的放射性碳比使用HNE时多十倍。一种特异性弹性蛋白酶抑制剂可显著减少PPE诱导的放射性碳释放。与[14C]TDI/PCL/ED相比，当[14C]TDI/PTMO/ED与PPE孵育时，释放的放射性碳少十倍。由于在炎症反应期间存在具有弹性溶解活性的中性粒细胞，植入装置中使用的生物材料的稳定性可能会受到影响。
