McMinn L H, Hodges G M, Carr K E
School of Biomedical Science/Anatomy, Queen's University of Belfast, Northern Ireland, UK.
J Anat. 1996 Dec;189 ( Pt 3)(Pt 3):553-9.
Uptake and translocation of particulates across the mucosal barrier of the gastrointestinal (GI) tract is now generally recognised but the effect of pathophysiologically induced changes on this process is less well established. This study evaluated the effect of diabetes mellitus on GI absorption of particles, comparing particle localisation and particle loading in different microanatomical sites of the primary organ (small intestine) and possible particle translocation pathways to selected secondary organs (mesenteric lymph nodes, liver, spleen) in normal and streptozotocin-induced diabetic animals. Fluorescent polystyrene latex particles (approximately 2 microns diameter) were fed orally to young adult Sprague-Dawley rats and quantitative bulk tissue and morphological techniques used to chart particle transit across the small intestine to secondary organs 0.5 h postadministration. In the normal animal, epifluorescence and confocal laser scanning microscopy provided confirmatory evidence for particle absorption within the primary organ and transport to other sites in the body. By contrast, in the diabetic animal, particle translocation and peripheral distribution were reduced with approximately 30% decrease in particle loading in the epithelial/nonepithelial tissue compartments. This could be a consequence of gastric retention and altered intestinal motility and permeability which are known to be associated with diabetes.
颗粒物质穿过胃肠道（GI）粘膜屏障的摄取和转运现已得到普遍认可，但病理生理诱导的变化对这一过程的影响尚不明确。本研究评估了糖尿病对颗粒物质胃肠道吸收的影响，比较了正常动物和链脲佐菌素诱导的糖尿病动物中，颗粒在主要器官（小肠）不同微观解剖部位的定位和负载情况，以及颗粒向选定二级器官（肠系膜淋巴结、肝脏、脾脏）的可能转运途径。将荧光聚苯乙烯乳胶颗粒（直径约2微米）经口投喂给年轻成年Sprague-Dawley大鼠，并使用定量整体组织和形态学技术绘制给药后0.5小时颗粒从小肠到二级器官的转运情况。在正常动物中，落射荧光和共聚焦激光扫描显微镜为颗粒在主要器官内的吸收以及向身体其他部位的转运提供了确凿证据。相比之下，在糖尿病动物中，颗粒转运和外周分布减少，上皮/非上皮组织隔室中的颗粒负载量减少了约30%。这可能是胃潴留以及肠道运动和通透性改变的结果，已知这些都与糖尿病有关。
