Arndt H, Bolanowski M A, Granger D N
Department of Physiology and Biophysics, Louisiana State University Medical Center, USA.
Gut. 1996 Jun;38(6):911-5. doi: 10.1136/gut.38.6.911.
An important action of interleukin 8 (IL8) is stimulation of granulocytes. The object of this study was to assess the contribution of IL8 to the leucocyte-endothelial cell interactions associated with intestinal inflammation in the rat.
Two indomethacin injections (48 and 24 hours prior to the experiments) induced a longlasting ileitis in rats. The number of adherent and emigrated leucocytes, leucocyte rolling velocity, and shear rate were monitored in normal and inflamed mesenteric postcapillary venules. Some animals received a monoclonal antibody (MAb) against IL8 or CD11b/CD18 at 24 and 12 hours prior to the experiment.
Indomethacin elicited a seven-fold increase in leucocyte adherence and a 5.4-fold increase in leucocyte emigration, while leucocyte rolling velocity was reduced by nearly 80%. The indomethacin induced increases in leucocyte adherence and emigration were significantly reduced (by 57% and 67%, respectively) while leucocyte rolling velocity was increased (to 63% of control) by the IL8-specific MAb. The level of inhibition seen with the IL8 MAb was similar to that associated with administration of a MAb directed against the leucocyte adhesion molecule CD11b/CD18.
IL8 contributes to the leucocyte-endothelial cell interactions elicited in mesenteric venules by indomethacin.
白细胞介素8（IL8）的一项重要作用是刺激粒细胞。本研究的目的是评估IL8在与大鼠肠道炎症相关的白细胞 - 内皮细胞相互作用中的作用。
在实验前48小时和24小时两次注射吲哚美辛可诱发大鼠持久的回肠炎。在正常和发炎的肠系膜毛细血管后微静脉中监测黏附及游出的白细胞数量、白细胞滚动速度和剪切率。部分动物在实验前24小时和12小时接受抗IL8或CD11b/CD18单克隆抗体（MAb）。
吲哚美辛使白细胞黏附增加7倍，白细胞游出增加5.4倍，而白细胞滚动速度降低近80%。IL8特异性单克隆抗体使吲哚美辛诱导的白细胞黏附和游出增加显著减少（分别减少57%和67%），同时白细胞滚动速度增加（达到对照的63%）。IL8单克隆抗体的抑制水平与针对白细胞黏附分子CD11b/CD18的单克隆抗体给药后的抑制水平相似。
IL8在吲哚美辛诱发的肠系膜微静脉白细胞 - 内皮细胞相互作用中起作用。
