Than S, Hu R, Oyaizu N, Romano J, Wang X, Sheikh S, Pahwa S
Department of Pediatrics, North Shore University Hospital-Cornell University Medical College, Manhasset, New York 11030, USA.
J Infect Dis. 1997 Jan;175(1):47-56. doi: 10.1093/infdis/175.1.47.
Cytokine mRNA expression and stimulus-induced cytokines were examined in peripheral blood mononuclear cells in 62 human immunodeficiency virus (HIV)-infected children and uninfected controls. Compared with that in controls, constitutive mRNA expression in patients was increased for tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, and interleukin (IL)-10 and decreased for IL-12; it was undetectable for IL-2 and IL-4 in both patients and controls. Stimulus-induced secretion of TNF-alpha, IFN-gamma, IL-12, and IL-4 was less than that in controls; IL-10 secretion was similar. There was no increase in stimulus-induced or constitutive IL-4 or IL-10 in children with severe immunologic deficit compared with controls. A higher stimulus-induced IL-10 secretion and a lower constitutive TNF-alpha mRNA were associated with a slower rate of disease progression, and TNF-alpha mRNA expression correlated with lower plasma HIV RNA. Thus, constitutive cytokine mRNA expression differs from stimulus-induced cytokine responses. The dominant defect in HIV-infected children appears to be one of reduced type 1 cytokines, predominantly IL-2.
在62名人类免疫缺陷病毒（HIV）感染儿童和未感染儿童的外周血单个核细胞中检测细胞因子mRNA表达及刺激诱导的细胞因子。与对照组相比，患者中肿瘤坏死因子（TNF）-α、干扰素（IFN）-γ和白细胞介素（IL）-10的组成型mRNA表达增加，而IL-12的表达降低；患者和对照组中IL-2和IL-4均未检测到。刺激诱导的TNF-α、IFN-γ、IL-12和IL-4分泌低于对照组；IL-10分泌相似。与对照组相比，严重免疫缺陷儿童中刺激诱导或组成型的IL-4或IL-10没有增加。刺激诱导的IL-10分泌较高和组成型TNF-α mRNA较低与疾病进展较慢相关，且TNF-α mRNA表达与较低的血浆HIV RNA相关。因此，组成型细胞因子mRNA表达与刺激诱导的细胞因子反应不同。HIV感染儿童的主要缺陷似乎是1型细胞因子减少，主要是IL-2。
