黄瓜花叶病毒卫星RNA的支持定位在辅助病毒解旋酶结构域附近的单个氨基酸上。
Support of a cucumber mosaic virus satellite RNA maps to a single amino acid proximal to the helicase domain of the helper virus.
作者信息
Roossinck M J, Kaplan I, Palukaitis P
机构信息
Plant Biology Division, The S. R. Noble Foundation, Ardmore, Oklahoma 73402, USA.
出版信息
J Virol. 1997 Jan;71(1):608-12. doi: 10.1128/JVI.71.1.608-612.1997.
Abstract
Cucumber mosaic virus (CMV) is a tripartite RNA virus that can support the replication of satellite RNAs, small molecular parasites of the virus. Satellite RNAs can have a dramatic effect on the helper virus and the host plant in a manner specific to the helper, satellite, and host. Previously, we showed that the Sny-CMV strain is not able to support the replication of the WL1 satellite RNA in zucchini squash and that this phenotype maps to RNA 1. In the present study, we use recombinant cDNA clones of Fny- and Sny-CMV RNA 1 and a site-directed mutant of Fny-CMV RNA 1 to demonstrate that the inability to support WL1 satellite RNA maps to a single amino acid at residue 978 in the 1a protein, proximal to the helicase domain VI. Support of satellite RNA in whole plants and in protoplasts of zucchini squash is analyzed.
摘要
黄瓜花叶病毒（CMV）是一种三分体RNA病毒，能够支持卫星RNA（该病毒的小分子寄生体）的复制。卫星RNA能够以一种依赖于辅助病毒、卫星RNA和宿主的方式，对辅助病毒和宿主植物产生显著影响。此前，我们发现Sny-CMV毒株无法支持WL1卫星RNA在西葫芦中的复制，且该表型定位于RNA 1。在本研究中，我们使用Fny-CMV和Sny-CMV RNA 1的重组cDNA克隆以及Fny-CMV RNA 1的一个定点突变体，来证明无法支持WL1卫星RNA的复制这一特性定位于1a蛋白中靠近解旋酶结构域VI的第978位氨基酸残基。我们分析了西葫芦整株植物和原生质体中卫星RNA的支持情况。
相似文献
1
Support of a cucumber mosaic virus satellite RNA maps to a single amino acid proximal to the helicase domain of the helper virus.黄瓜花叶病毒卫星RNA的支持定位在辅助病毒解旋酶结构域附近的单个氨基酸上。
J Virol. 1997 Jan;71(1):608-12. doi: 10.1128/JVI.71.1.608-612.1997.
2
Differential effects of satellite RNA on the accumulation of cucumber mosaic virus RNAs and their encoded proteins in tobacco vs zucchini squash with two strains of CMV helper virus.卫星RNA对两种黄瓜花叶病毒（CMV）辅助病毒株在烟草和西葫芦中黄瓜花叶病毒RNA积累及其编码蛋白的差异影响。
Virology. 1995 Apr 1;208(1):58-66. doi: 10.1006/viro.1995.1129.
3
Differential replication in zucchini squash of a cucumber mosaic virus satellite RNA maps to RNA 1 of the helper virus.黄瓜花叶病毒卫星RNA在西葫芦中的差异复制定位于辅助病毒的RNA 1。
Virology. 1991 Mar;181(1):371-3. doi: 10.1016/0042-6822(91)90506-7.
4
Genetic analysis of helper virus-specific selective amplification of cucumber mosaic virus satellite RNAs.
J Mol Evol. 1995 Jan;40(1):25-9. doi: 10.1007/BF00166593.
5
Variation in the hypervariable region of cucumber mosaic virus satellite RNAs is affected by the helper virus and the initial sequence context.
Virology. 1995 Jan 10;206(1):765-8. doi: 10.1016/s0042-6822(95)80006-9.
6
Mapping helper virus functions for cucumber mosaic virus satellite RNA with pseudorecombinants derived from cucumber mosaic and tomato aspermy viruses.利用源自黄瓜花叶病毒和番茄不孕病毒的假重组体对黄瓜花叶病毒卫星RNA的辅助病毒功能进行定位
Virology. 1994 Dec;205(2):574-7. doi: 10.1006/viro.1994.1682.
7
The complete sequence of a cucumber mosaic virus from Ixora that is deficient in the replication of satellite RNAs.一种来自龙船花属植物的黄瓜花叶病毒的完整序列，该病毒在卫星RNA复制方面存在缺陷。
J Gen Virol. 1995 Sep;76 ( Pt 9):2257-70. doi: 10.1099/0022-1317-76-9-2257.
8
The kinetics of infection of zucchini squash by cucumber mosaic virus indicate a function for RNA 1 in virus movement.
Virology. 1994 Nov 15;205(1):280-9. doi: 10.1006/viro.1994.1644.
9
Involvement of RNA2 for systemic infection of Cucumber mosaic virus isolated from lily on zucchini squash.RNA2在从百合分离的黄瓜花叶病毒对西葫芦的系统感染中的作用。
Virus Res. 2003 Nov;97(1):1-6. doi: 10.1016/s0168-1702(03)00215-6.
10
Helicase domain encoded by Cucumber mosaic virus RNA1 determines systemic infection of Cmr1 in pepper.由黄瓜花叶病毒 RNA1 编码的解旋酶结构域决定了 Cmr1 在辣椒中的系统性感染。
PLoS One. 2012;7(8):e43136. doi: 10.1371/journal.pone.0043136. Epub 2012 Aug 15.
引用本文的文献
1
Phylogeography and Coevolution of Bamboo Mosaic Virus and Its Associated Satellite RNA.竹花叶病毒及其相关卫星RNA的系统发育地理学与协同进化
Front Microbiol. 2017 May 23;8:886. doi: 10.3389/fmicb.2017.00886. eCollection 2017.
2
Mapping viral functional domains for genetic diversity in plants.植物遗传多样性的病毒功能域作图。
J Virol. 2013 Jan;87(2):790-7. doi: 10.1128/JVI.01891-12. Epub 2012 Oct 31.
3
Fixation of emerging interviral recombinants in cucumber mosaic virus populations.新兴间质重组体在黄瓜花叶病毒群体中的固定。
J Virol. 2013 Jan;87(2):1264-9. doi: 10.1128/JVI.01892-12. Epub 2012 Oct 31.
4
Helicase domain encoded by Cucumber mosaic virus RNA1 determines systemic infection of Cmr1 in pepper.由黄瓜花叶病毒 RNA1 编码的解旋酶结构域决定了 Cmr1 在辣椒中的系统性感染。
PLoS One. 2012;7(8):e43136. doi: 10.1371/journal.pone.0043136. Epub 2012 Aug 15.
5
Environment determines fidelity for an RNA virus replicase.环境决定RNA病毒复制酶的保真度。
J Virol. 2007 Sep;81(17):9072-7. doi: 10.1128/JVI.00587-07. Epub 2007 Jun 6.
6
Genetic bottlenecks reduce population variation in an experimental RNA virus population.基因瓶颈减少了实验性RNA病毒群体中的种群变异。
J Virol. 2004 Oct;78(19):10582-7. doi: 10.1128/JVI.78.19.10582-10587.2004.
7
Cucumber mosaic virus D satellite RNA-induced programmed cell death in tomato.黄瓜花叶病毒D卫星RNA诱导番茄细胞程序性死亡
Plant Cell. 2000 Jul;12(7):1079-92. doi: 10.1105/tpc.12.7.1079.
8
Evolutionarily related Sindbis-like plant viruses maintain different levels of population diversity in a common host.进化相关的辛德毕斯样植物病毒在共同宿主中维持着不同程度的种群多样性。
J Virol. 2000 Apr;74(7):3130-4. doi: 10.1128/jvi.74.7.3130-3134.2000.
本文引用的文献
1
Differential effects of satellite RNA on the accumulation of cucumber mosaic virus RNAs and their encoded proteins in tobacco vs zucchini squash with two strains of CMV helper virus.卫星RNA对两种黄瓜花叶病毒（CMV）辅助病毒株在烟草和西葫芦中黄瓜花叶病毒RNA积累及其编码蛋白的差异影响。
Virology. 1995 Apr 1;208(1):58-66. doi: 10.1006/viro.1995.1129.
2
A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement.一种由植物RNA病毒编码的新型天然杂交基因促进病毒长距离移动。
EMBO J. 1995 Dec 1;14(23):5762-72. doi: 10.1002/j.1460-2075.1995.tb00265.x.
3
The kinetics of infection of zucchini squash by cucumber mosaic virus indicate a function for RNA 1 in virus movement.
Virology. 1994 Nov 15;205(1):280-9. doi: 10.1006/viro.1994.1644.
4
Characterization of deletion and frameshift mutants of satellite tobacco mosaic virus.烟草花叶病毒卫星病毒缺失和移码突变体的特征分析
Virology. 1995 Sep 10;212(1):121-7. doi: 10.1006/viro.1995.1460.
5
Characterization of defective RNAs derived from RNA 3 of the Fny strain of cucumber mosaic cucumovirus.黄瓜花叶黄瓜病毒Fny株系RNA 3衍生的缺陷RNA的特性分析
J Virol. 1995 Aug;69(8):4746-51. doi: 10.1128/JVI.69.8.4746-4751.1995.
6
Analysis of the determinants of the satellite RNA of cucumber mosaic cucumovirus for high accumulation in squash.黄瓜花叶黄瓜病毒卫星RNA在南瓜中高积累的决定因素分析。
Virology. 1994 Nov 15;205(1):262-8. doi: 10.1006/viro.1994.1642.
7
An amino acid sequence motif linking viral DNA polymerases and plant virus proteins involved in RNA replication.一种连接病毒DNA聚合酶与参与RNA复制的植物病毒蛋白的氨基酸序列基序。
Nucleic Acids Res. 1986 Aug 26;14(16):6769. doi: 10.1093/nar/14.16.6769.
8
A new superfamily of replicative proteins.一个新的复制蛋白超家族。
Nature. 1988 May 5;333(6168):22-3. doi: 10.1038/333022b0.
9
A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.DNA片段的体外制备及特异性诱变的通用方法：蛋白质与DNA相互作用的研究
Nucleic Acids Res. 1988 Aug 11;16(15):7351-67. doi: 10.1093/nar/16.15.7351.
10
Evolutionary relationship between luteoviruses and other RNA plant viruses based on sequence motifs in their putative RNA polymerases and nucleic acid helicases.基于黄症病毒属病毒及其它RNA植物病毒假定的RNA聚合酶和核酸解旋酶中的序列基序，研究它们之间的进化关系。
Nucleic Acids Res. 1989 Dec 11;17(23):9543-55. doi: 10.1093/nar/17.23.9543.
Zotero 插件