Roossinck M J, Kaplan I, Palukaitis P
Plant Biology Division, The S. R. Noble Foundation, Ardmore, Oklahoma 73402, USA.
J Virol. 1997 Jan;71(1):608-12. doi: 10.1128/JVI.71.1.608-612.1997.
Cucumber mosaic virus (CMV) is a tripartite RNA virus that can support the replication of satellite RNAs, small molecular parasites of the virus. Satellite RNAs can have a dramatic effect on the helper virus and the host plant in a manner specific to the helper, satellite, and host. Previously, we showed that the Sny-CMV strain is not able to support the replication of the WL1 satellite RNA in zucchini squash and that this phenotype maps to RNA 1. In the present study, we use recombinant cDNA clones of Fny- and Sny-CMV RNA 1 and a site-directed mutant of Fny-CMV RNA 1 to demonstrate that the inability to support WL1 satellite RNA maps to a single amino acid at residue 978 in the 1a protein, proximal to the helicase domain VI. Support of satellite RNA in whole plants and in protoplasts of zucchini squash is analyzed.
黄瓜花叶病毒（CMV）是一种三分体RNA病毒，能够支持卫星RNA（该病毒的小分子寄生体）的复制。卫星RNA能够以一种依赖于辅助病毒、卫星RNA和宿主的方式，对辅助病毒和宿主植物产生显著影响。此前，我们发现Sny-CMV毒株无法支持WL1卫星RNA在西葫芦中的复制，且该表型定位于RNA 1。在本研究中，我们使用Fny-CMV和Sny-CMV RNA 1的重组cDNA克隆以及Fny-CMV RNA 1的一个定点突变体，来证明无法支持WL1卫星RNA的复制这一特性定位于1a蛋白中靠近解旋酶结构域VI的第978位氨基酸残基。我们分析了西葫芦整株植物和原生质体中卫星RNA的支持情况。
