黄瓜花叶黄瓜病毒Fny株系RNA 3衍生的缺陷RNA的特性分析
Characterization of defective RNAs derived from RNA 3 of the Fny strain of cucumber mosaic cucumovirus.
作者信息
Graves M V, Roossinck M J
机构信息
Plant Biology Division, Samuel Roberts Noble Foundation, Inc., Ardmore, Oklahoma 73402-2180, USA.
出版信息
J Virol. 1995 Aug;69(8):4746-51. doi: 10.1128/JVI.69.8.4746-4751.1995.
Abstract
Two defective RNAs (designated D RNA 3 alpha and D RNA 3 beta) were found to be associated with the Fny strain of cucumber mosaic cucumovirus but not with the Sny strain after serial passages in a tobacco host. The D RNAs were derived from RNA 3 by single, in-frame deletions within the 3a open reading frame. A full-length cDNA clone from which biologically active transcripts can be produced in vitro has been constructed for D RNA 3 beta. This transcript can be replicated in tobacco plants infected with subgroup I and II cucumber mosaic cucumovirus strains and with peanut stunt cucumovirus. Translation of D RNA 3 beta in vitro produced a 20-kDa peptide, which was consistent with the predicted coding capacity of the deleted 3a open reading frame. D RNA 3 beta was also associated with polyribosomes isolated from infected tobacco plants. The presence of the D RNAs had no apparent effect upon helper virus yield or symptom production.
摘要
在烟草宿主中连续传代后，发现两种缺陷型RNA（命名为D RNA 3α和D RNA 3β）与黄瓜花叶黄瓜病毒的Fny株系相关，但与Sny株系无关。这些D RNA是由RNA 3通过3a开放阅读框内的单个读框内缺失产生的。已经构建了一个全长cDNA克隆，可在体外产生具有生物活性的转录本用于D RNA 3β。该转录本可在感染I组和II组黄瓜花叶黄瓜病毒株系以及花生矮化黄瓜病毒的烟草植株中复制。D RNA 3β在体外翻译产生了一个20 kDa的肽，这与缺失的3a开放阅读框的预测编码能力一致。D RNA 3β也与从感染烟草植株中分离的多核糖体相关。D RNA的存在对辅助病毒产量或症状产生没有明显影响。
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