Priel M R, dos Santos N F, Cavalheiro E A
Neurologia Experimental, UNIFESP-EPM, São Paulo, Brazil.
Epilepsy Res. 1996 Dec;26(1):115-21. doi: 10.1016/s0920-1211(96)00047-2.
Prolonged seizures in young children may precede the later development of focal or generalized seizures. To study the age-related susceptibility to the development of chronic epilepsy we used the pilocarpine model of epilepsy (PME). This model is well characterized in adult rats, and presents three distinct periods: (a) an acute period of status epilepticus (SE), (b) a silent period of a progressive normalization of EEG and behavior, (c) a chronic period of spontaneous recurrent seizures. Wistar rats aged 7-120 days received pilocarpine hydrochloride (170-380 mg/kg, i.p., according to age), 30 min after methylscopolamine (1 mg/kg, s.c.). All surviving animals were observed for 120 days. The results indicate that chronic seizures following pilocarpine-induced status epilepticus can be induced in rats if the status is induced after the 18th day of life. The age-related differences in the susceptibility of young rats to developed chronic epilepsy reflect the complexity of seizure activity in immature brain and provide for an apparent distinction between the mechanisms of epileptogenesis in the mature and developing nervous system.
幼儿期的长时间癫痫发作可能先于局灶性或全身性癫痫的后期发展。为了研究与年龄相关的慢性癫痫发展易感性，我们使用了毛果芸香碱癫痫模型（PME）。该模型在成年大鼠中特征明确，呈现三个不同阶段：（a）癫痫持续状态（SE）急性期，（b）脑电图和行为逐渐恢复正常的静止期，（c）自发性反复癫痫发作的慢性期。7至120日龄的Wistar大鼠在皮下注射甲基东莨菪碱（1mg/kg）30分钟后，腹腔注射盐酸毛果芸香碱（170 - 380mg/kg，根据年龄）。观察所有存活动物120天。结果表明，如果在出生后第18天之后诱导癫痫持续状态，毛果芸香碱诱导的癫痫持续状态后慢性癫痫发作可在大鼠中诱发。幼鼠对慢性癫痫发展易感性的年龄相关差异反映了未成熟大脑中癫痫活动的复杂性，并在成熟和发育中的神经系统癫痫发生机制之间提供了明显区别。
