Diemer T, Weidner W, Michelmann H W, Schiefer H G, Rovan E, Mayer F
Department of Urology, University Hospital, Göttingen, Germany.
Int J Androl. 1996 Oct;19(5):271-7. doi: 10.1111/j.1365-2605.1996.tb00475.x.
The influence of E. coli on human sperm motility was studied in vitro. Semen samples were prepared by a swim-up technique and adjusted to 22 x 10(6) spermatozoa/ml. Samples were then inoculated with different concentrations of a uropathogenic strain of E. coli, serotype 06, with initial sperm/bacteria ratios varying between 10:1 and 10000:1. Motion parameters were analysed by computer-aided motility analysis directly, and 2, 4 and 6 h after inoculation. In a second series of experiments, bacterial replication was inhibited by addition of chloramphenicol. In a third series, the effect of E. coli culture filtrates on sperm motility was investigated. The direct inhibitory effect of E. coli on progressive motility of spermatozoa was found to depend upon the bacterial concentration. A distinct inhibitory effect was observed only at a sperm/bacteria ratio of approximately 1, achieved by growth of E. coli during the experiments. For modality of motion, no distinct changes were observed. When growth of bacteria was prevented by chloramphenicol, no inhibitory effect on sperm motility was detected. Sperm motility was not inhibited by E. coli culture filtrates. Analysis by electron microscopy revealed multiple adhesions of E. coli to spermatozoa, causing variable ultrastructural damage as probable morphological correlates of immobilization.
在体外研究了大肠杆菌对人类精子活力的影响。精液样本通过上浮技术制备，并调整至22×10⁶精子/毫升。然后将样本接种不同浓度的致病性大肠杆菌06血清型菌株，初始精子/细菌比例在10:1至10000:1之间变化。在接种后直接以及接种后2、4和6小时，通过计算机辅助活力分析对运动参数进行分析。在第二系列实验中，通过添加氯霉素抑制细菌复制。在第三系列实验中，研究了大肠杆菌培养滤液对精子活力的影响。发现大肠杆菌对精子渐进性活力的直接抑制作用取决于细菌浓度。仅在实验期间大肠杆菌生长达到的约1的精子/细菌比例时观察到明显的抑制作用。对于运动方式，未观察到明显变化。当用氯霉素阻止细菌生长时，未检测到对精子活力的抑制作用。大肠杆菌培养滤液未抑制精子活力。电子显微镜分析显示大肠杆菌与精子有多处粘连，导致可变的超微结构损伤，这可能是固定化的形态学相关因素。
