Diemer T, Huwe P, Ludwig M, Schroeder-Printzen I, Michelmann H W, Schiefer H G, Weidner W
Department of Urology, University Hospital Giessen, Justus-Liebig-Universität Giessen, Klinikstrasse 29, 35385 Giessen, Germany.
Andrologia. 2003 Apr;35(2):100-5. doi: 10.1046/j.1439-0272.2003.00523.x.
Urogenital infections are considered important factors in male infertility. In this in vitro study we have evaluated the impact of leucocytes in association with an artificial infection with Escherichia coli on the motility of human spermatozoa. Ejaculates and blood samples were obtained from healthy donors with normal semen parameters. Ejaculates were prepared by swim-up technique and five fractions were isolated for incubation. Leucocyte subtypes were separated from blood samples by gradient centrifugation. Purified sperm suspensions were adjusted to a concentration of 20 x 106 ml-1 and incubated with lymphocytes/ monocytes, polymorphonuclear granulocytes (PMN), and E. coli. Samples were incubated for up to 6 h at 37 degrees C. Motility analysis was performed using a computer-assisted sperm analyzer (CASA). Spermatozoa incubated with 3 x 106 PMN ml-1 revealed a significant (P=0.003) decrease in progressive motility after 2 h. This decrease remained weakly significant (P=0.024) after 4 and 6 h. Lymphocytes and monocytes had no effect on sperm motility. Spermatozoa incubated with granulocytes and E. coli demonstrated highly significant alterations in motility after 4 and 6 h of incubation (P < 0.001). The PMN indicate an effect on motility of spermatozoa under experimental conditions. However, the results suggest that bacteria are the primary agents that interfere with sperm motility.
泌尿生殖系统感染被认为是男性不育的重要因素。在这项体外研究中，我们评估了白细胞与大肠杆菌人工感染联合作用对人类精子活力的影响。从精液参数正常的健康供体获取精液和血液样本。通过上浮技术制备精液，并分离出五个部分用于孵育。通过梯度离心从血液样本中分离白细胞亚型。将纯化的精子悬液调整至浓度为20×10⁶/ml，并与淋巴细胞/单核细胞、多形核粒细胞（PMN）和大肠杆菌一起孵育。样本在37℃下孵育长达6小时。使用计算机辅助精子分析仪（CASA）进行活力分析。与3×10⁶个PMN/ml孵育的精子在2小时后显示出进行性活力显著下降（P = 0.003）。在4小时和6小时后，这种下降仍具有微弱的显著性（P = 0.024）。淋巴细胞和单核细胞对精子活力没有影响。与粒细胞和大肠杆菌一起孵育的精子在孵育4小时和6小时后显示出活力的高度显著变化（P < 0.001）。PMN在实验条件下表明对精子活力有影响。然而，结果表明细菌是干扰精子活力的主要因素。
