在日本硬蜱中发现的新种田鼠疏螺旋体和新种松鸡疏螺旋体：通过靶向16S rRNA基因的PCR分析进行快速物种鉴定。
Borrelia tanukii sp. nov. and Borrelia turdae sp. nov. found from ixodid ticks in Japan: rapid species identification by 16S rRNA gene-targeted PCR analysis.
作者信息
Fukunaga M, Hamase A, Okada K, Nakao M
机构信息
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan.
出版信息
Microbiol Immunol. 1996;40(11):877-81. doi: 10.1111/j.1348-0421.1996.tb01154.x.
Based on the results of RFLP-ribotyping, whole DNA/DNA hybridization and phylogenetic analysis of the 16S rRNA gene, we previously defined two genomic groups of spirochetes closely related to Borrelia burgdorferi sensu lato: group Hk501 for strains isolated from Ixodes tanuki ticks and group Ya501 for strains isolated from Ixodes turdus ticks. In this report, we propose that group Hk501 should be classified as Borrelia tanukii sp. nov. and group Ya501 as Borrelia turdae sp. nov. The alignment of previously published Borrelia 16S rRNA gene sequences led us to design species-specific PCR primer sets. The primers allowed the rapid identification of B. tanukii and B. turdae.
基于限制性片段长度多态性核糖体分型、全DNA/DNA杂交以及16S rRNA基因的系统发育分析结果，我们之前定义了与狭义伯氏疏螺旋体密切相关的两个螺旋体基因组：从狸蜱中分离出的菌株组成的Hk501组，以及从乌鸫蜱中分离出的菌株组成的Ya501组。在本报告中，我们提议将Hk501组分类为新种田鼠伯氏疏螺旋体（Borrelia tanukii），将Ya501组分类为新种乌鸫伯氏疏螺旋体（Borrelia turdae）。对先前发表的伯氏疏螺旋体16S rRNA基因序列进行比对后，我们设计了种特异性PCR引物组。这些引物能够快速鉴定田鼠伯氏疏螺旋体和乌鸫伯氏疏螺旋体。
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