Filev F
Vet Med Nauki. 1977;14(1):74-8.
Studied were a total of 16,571 cows on 89 farms by means of the Bernburg test. Milk was sampled from the positively reacting quarters of the udder by taking 18,047 samples intended for bacteriologic investigation. The demonstration of mastitis streptococci was carried out on "TKT" agar Merk, of pathogenic staphylococci, hemolytic streptococci, and Corinebacteria--on dextrose agar Oxoid containing 7.5% citrated calf blood. The isolated hemolytic streptococcus bacteria from the two nutrient media were differentiated through the CAMP test as well as serologically by the precipitation agar gel and Difco sera. The pathogenicity of Staphylococcus bacteria, in addition through hemolysis, was tested by the use of plasma coagulase with citrated rabbit plasma. In 53.95% of the cases there were secretory lesions due to Sc. agalactiae (6.23%) to Sc. dysgalactiae (5.69%) to Sc. uberis (8.47%), to Staph. aureus (2.44%), to hemolytic streptococci of the C, G and L groups (0.28%), to Sc. viridans (0.03%), to Corynebacterium pyogenes (0.41%), and catarrhal mastitis (30.4%). Some of the causative agents, such as Sc. agalactiae, Staph. aureus, and others have proved of epidemiologic importance to humans.
通过伯恩堡试验对89个农场的16571头奶牛进行了研究。从乳房呈阳性反应的乳区采集了18047份用于细菌学调查的样本。乳腺炎链球菌的检测在默克公司的“TKT”琼脂上进行,致病性葡萄球菌、溶血性链球菌和棒状杆菌的检测在含有7.5%柠檬酸盐小牛血的奥克托德葡萄糖琼脂上进行。从两种培养基中分离出的溶血性链球菌通过CAMP试验以及沉淀琼脂凝胶和迪夫科血清进行血清学鉴别。葡萄球菌的致病性除通过溶血外,还通过使用柠檬酸盐兔血浆的血浆凝固酶进行检测。在53.95%的病例中,存在由无乳链球菌(6.23%)、停乳链球菌(5.69%)、乳房链球菌(8.47%)、金黄色葡萄球菌(2.44%)、C、G和L组溶血性链球菌(0.28%)、绿色链球菌(0.03%)、化脓棒状杆菌(0.41%)引起的分泌性病变以及卡他性乳腺炎(30.4%)。一些病原体,如无乳链球菌、金黄色葡萄球菌等已被证明对人类具有流行病学重要性。