Yeung W S, Lau E Y, Chan A Y, Ho P C
Department of Obstetrics and Gynaecology, University of Hong Kong, Queen Mary Hospital, Hong Kong.
J Assist Reprod Genet. 1996 Nov;13(10):772-5. doi: 10.1007/BF02066496.
The aim of this study was to determine the concentration of interleukin-1 alpha in human embryo culture medium with or without oviductal cell coculture and to correlate the interleukin-1 alpha levels with pregnancy.
Culture media from 32 in vitro fertilization and embryo transfer cycles were assayed for interleukin-1 alpha by immunoassay technique. Human embryos were cultured in Earles' balanced salt solution supplemented with 15% preovulatory serum (sEBSS) in 16 of these cycles, while embryos in the rest of the cycles were cocultured with human oviductal cells in sEBSS.
Both sEBSS and spent sEBSS after embryo culture contained low or undetectable levels of interleukin-1 alpha in the pregnant and nonpregnant cycles. On the other hand, oviductal cells significantly increased the amount of interleukin-1 alpha immunoreactivity in the conventional culture medium or coculture medium (P < 0.001, Mann-Whitney rank sum test). The concentrations of interleukin-1 alpha in the spent sEBSS after oviductal cell culture and after coculture with human embryos were 1.5 +/- 1.0 and 1.3 +/- 0.9 pg/ml, respectively. There was no difference in the interleukin-1 alpha concentration between the pregnant and the nonpregnant coculture cycles.
These data showed that human oviductal cells produced interleukin-1 alpha immunoreactivity in a coculture system. However, this production could not be used as a marker for successful embryo implantation.
本研究的目的是测定在有或无输卵管细胞共培养的情况下人胚胎培养基中白细胞介素-1α的浓度，并将白细胞介素-1α水平与妊娠情况相关联。
采用免疫测定技术对32个体外受精和胚胎移植周期的培养基进行白细胞介素-1α检测。在其中16个周期中，人胚胎在补充有15%排卵前血清的Earles平衡盐溶液（sEBSS）中培养，而其余周期的胚胎则在sEBSS中与人输卵管细胞进行共培养。
在妊娠和非妊娠周期中，胚胎培养后的sEBSS和用过的sEBSS中白细胞介素-1α水平均较低或检测不到。另一方面，输卵管细胞显著增加了传统培养基或共培养基中白细胞介素-1α免疫反应性的量（P<0.001，Mann-Whitney秩和检验）。输卵管细胞培养后以及与人胚胎共培养后用过的sEBSS中白细胞介素-1α的浓度分别为1.5±1.0和1.3±0.9 pg/ml。妊娠和非妊娠共培养周期之间白细胞介素-1α浓度没有差异。
这些数据表明，在共培养系统中，人输卵管细胞产生白细胞介素-1α免疫反应性。然而，这种产生不能用作胚胎成功着床的标志物。
