Coutlée F, Hankins C, Lapointe N
Départment de Microbiologie-Immunologie, Université de Montréal, Québec, Canada.
J Med Virol. 1997 Jan;51(1):42-7. doi: 10.1002/(sici)1096-9071(199701)51:1<42::aid-jmv7>3.0.co;2-s.
The aim of the study was to compare the accuracy of self-administered vaginal tampon (VT) specimens for the detection of human papillomaviruses (HPVs) with that of cervicovaginal lavage specimens (CVL). Two hundred seventy-four paired VT and CVL specimens were collected prospectively from women at risk of sexually transmitted diseases. Specimens were treated and amplified with the polymerase chain reaction (PCR). Each woman served as her own control. One hundred and forty-four of 272 (52.9%) CVLs and 159 of 271 (58.7%) VTs contained HPV DNA sequences (correlation of 88%). The sensitivity and specificity of vaginal tampons reached 93.9% (138/147) and 80.5% (99/123), respectively. HPV typing results were concordant for 99 negative paired samples and 114 paired samples positive for the same type(s) (correlation of 78.9%). It is concluded that these sampling methods collect cells from different areas of the genital epithelium, highlighting the importance of further assessment of the comparative predictive value of HPV detection in each sample.
本研究的目的是比较自我采集的阴道棉塞（VT）标本与宫颈阴道灌洗标本（CVL）在检测人乳头瘤病毒（HPV）方面的准确性。前瞻性地收集了274对来自有性传播疾病风险女性的VT和CVL标本。标本经聚合酶链反应（PCR）处理和扩增。每位女性都作为自己的对照。272份CVL标本中有144份（52.9%）以及271份VT标本中有159份（58.7%）含有HPV DNA序列（相关性为88%）。阴道棉塞的敏感性和特异性分别达到93.9%（138/147）和80.5%（99/123）。99对阴性配对样本以及114对相同类型阳性配对样本的HPV分型结果一致（相关性为78.9%）。结论是，这些采样方法从生殖器上皮的不同区域采集细胞，凸显了进一步评估每个样本中HPV检测的比较预测价值的重要性。
