York S W, Ingram L O
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611, USA.
J Ind Microbiol. 1996 Jun;16(6):374-6. doi: 10.1007/BF01570119.
An optimized soy-based medium was developed for ethanol production by Escherichia coli KO11. The medium consists of mineral salts, vitamins, crude enzymatic hydrolysate of soy and fermentable sugar. Ethanol produced after 24 h was used as an endpoint in bioassays to optimize hydrolysate preparation. Although longer fermentation times were required with soy medium than with LB medium, similar final ethanol concentrations were achieved (44-45 g ethanol L-1 from 100 g glucose L-1). The cost of materials for soy medium (excluding sugar) was estimated to be $0.003 L-1 broth, $0.06 L-1 ethanol.
开发了一种优化的大豆基培养基，用于大肠杆菌KO11生产乙醇。该培养基由矿物盐、维生素、大豆粗酶解物和可发酵糖组成。24小时后产生的乙醇用作生物测定中的终点，以优化水解产物的制备。虽然大豆培养基所需的发酵时间比LB培养基长，但最终乙醇浓度相似（从100 g/L葡萄糖中获得44 - 45 g/L乙醇）。大豆培养基（不包括糖）的材料成本估计为每升肉汤0.003美元，每升乙醇0.06美元。
