Department of Chemical and Biomolecular Engineering (BK21 program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Bioresour Technol. 2011 Aug;102(16):7466-9. doi: 10.1016/j.biortech.2011.04.071. Epub 2011 Apr 24.
Algae biomass is a potential raw material for the production of biofuels and other chemicals. In this study, biomass of the marine algae, Ulva lactuca, Gelidium amansii,Laminaria japonica, and Sargassum fulvellum, was treated with acid and commercially available hydrolytic enzymes. The hydrolysates contained glucose, mannose, galactose, and mannitol, among other sugars, at different ratios. The Laminaria japonica hydrolysate contained up to 30.5% mannitol and 6.98% glucose in the hydrolysate solids. Ethanogenic recombinant Escherichia coli KO11 was able to utilize both mannitol and glucose and produced 0.4g ethanol per g of carbohydrate when cultured in L. japonica hydrolysate supplemented with Luria-Bertani medium and hydrolytic enzymes. The strategy of acid hydrolysis followed by simultaneous enzyme treatment and inoculation with E. coli KO11 could be a viable strategy to produce ethanol from marine alga biomass.
藻类生物质是生产生物燃料和其他化学品的潜在原料。在这项研究中，使用酸和市售的水解酶处理了海洋藻类，如浒苔、石花菜、裙带菜和羊栖菜的生物质。水解产物中含有葡萄糖、甘露糖、半乳糖和甘露醇等不同比例的糖。在海带水解产物固体中，甘露醇含量高达 30.5%，葡萄糖含量为 6.98%。产乙醇重组大肠杆菌 KO11 能够利用甘露醇和葡萄糖，当在添加了 LB 培养基和水解酶的海带水解产物中培养时，每克碳水化合物可产生 0.4 克乙醇。酸水解后进行同时酶处理并用大肠杆菌 KO11 接种的策略可能是从海洋藻类生物质生产乙醇的可行策略。
