Hymer W C, Salada T, Cenci R, Krishnan K, Seaman G V, Snyder R, Matsumiya H, Nagaoka S
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802, USA.
J Biotechnol. 1996 Jun 27;47(2-3):353-65. doi: 10.1016/0168-1656(96)01406-x.
In this report we describe the results of a continuous flow electrophoresis (CFE) experiment done on STS-65 in which we tested the idea that intracellular growth hormone (GH) particles contained in a cell lysate prepared from cultured rat anterior pituitary cells in microgravity might have different electrophoretic mobilities from those in a synchronous ground control cell lysate. Collectively, the results suggested that CFE processing in microgravity was better than on earth; more sample could be processed/time (6 x) and more variant forms of GH molecules could be resolved as well. We had also hoped to carry out a pituitary cell CFE experiment, but failure of the hardware required that the actual cell electrophoresis trials be done on earth shortly after Shuttle landing. Data from these experiments showed that space-flown cells possessed a higher electrophoretic mobility than ground control cells, thereby offering evidence for the idea that exposure of cultured cells to microgravity can change their net surface charge-density especially when the cells are fed. Collectively, the results from this pituitary cell experiment document the advantage of using coupled cell culture and CFE techniques in the microgravity environment.
在本报告中，我们描述了在STS - 65上进行的连续流电泳（CFE）实验的结果。在该实验中，我们测试了这样一种想法：在微重力条件下，由培养的大鼠垂体前叶细胞制备的细胞裂解物中所含的细胞内生长激素（GH）颗粒，其电泳迁移率可能与同步地面对照细胞裂解物中的不同。总体而言，结果表明微重力条件下的CFE处理比在地球上更好；每单位时间可处理更多样本（多6倍），并且还能分辨出更多不同形式的GH分子。我们原本还希望进行垂体细胞CFE实验，但硬件出现故障，导致实际的细胞电泳试验只能在航天飞机着陆后不久在地球上进行。这些实验的数据表明，太空飞行的细胞比地面对照细胞具有更高的电泳迁移率，从而为以下观点提供了证据：培养的细胞暴露于微重力环境中会改变其净表面电荷密度，尤其是在给细胞喂食时。总体而言，该垂体细胞实验的结果证明了在微重力环境中使用细胞培养和CFE耦合技术的优势。
