Kobayashi H, Ishii N, Nagaoka S
Faculty of Science, JOSAI University, Sakado-Shi, Japan.
J Biotechnol. 1996 Jun 27;47(2-3):367-76. doi: 10.1016/0168-1656(96)01411-3.
Free flow electrophoresis of a nematode C. elegans DNA was carried out on the space shuttle flight STS65/Colombia. During the processes of the experiment, the house keeping data of the FFEU and the electrophoretic migration profiles were monitored at POCC (Payload Operations Control Center) of MSFC (Marshall Space Flight Center, Alabama) according to the real-time down-link system. The three dimensional electropherogram (3DEP) on the basis of the down-linked data showed some trouble with the apparatus but three sequential experiments indicated this disturbance of the apparatus is rather preferably stable. Comparing post-flight analyses of the DNA component fractionated, that is, amplification by PCR method, it revealed that the DNAs were separated approximately into two peaks: one of them contained seven-fold higher content of DNA estimated by a sod-4 gene probe than an unc-6 gene probe. These results suggested that this separation technique could be still more effective for the separation of biological macromolecules such as DNA, and the efficiency of separation of the free flow electrophoresis under microgravity environment was useful.
秀丽隐杆线虫DNA的自由流动电泳是在航天飞机STS65/哥伦比亚号的飞行任务中进行的。在实验过程中，根据实时下行链路系统，在阿拉巴马州马歇尔航天飞行中心的有效载荷操作控制中心（POCC）监测自由流动电泳装置（FFEU）的运行数据和电泳迁移图谱。基于下行链路数据的三维电泳图（3DEP）显示该装置存在一些问题，但连续三次实验表明该装置的这种干扰相当稳定。对分离的DNA组分进行飞行后分析，即通过PCR方法进行扩增，结果显示DNA大致分离为两个峰：其中一个峰中，通过sod - 4基因探针估计的DNA含量比unc - 6基因探针高七倍。这些结果表明，这种分离技术对于分离DNA等生物大分子可能更有效，并且微重力环境下自由流动电泳的分离效率是有用的。
